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Downregulated expression of hepatoma-derived growth factor (HDGF) reduces gallbladder cancer cell proliferation and invasion

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Abstract

Hepatoma-derived growth factor (HDGF), a heparin-binding growth factor, has a wide range of biological functions, including mitogenic activity and vascular development. Recent studies demonstrated that HDGF also acted as an oncogene with aberrantly increased activity in multiple human cancers; however, little is known about the biological function of HDGF in gallbladder cancer (GBC). In this study, we focused on the clinical significance and biological functions of HDGF in GBC and found that Nuclear HDGF protein overexpression was frequently detected in GBC tissues. Patients with nuclear HDGF-positive tumors had worse overall survival than patients with HDGF-negative tumors. Furthermore, treatment of GBC lines with HDGF-targeting siRNA oligonucleotides (HDGF-siRNA) significantly reduced the proliferation of GBC-SD and SGC-996 cell lines and diminished both anchorage-independent growth on soft agar and cell migration. These data indicate that HDGF acts as a putative oncogene in GBC and could be a novel diagnostic and therapeutic target for GBC.

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Acknowledgments

This work was supported by the Shanghai Committee of Science and Technology (No. 12JC1406700, 12410705900, 12401905800), the Foundation of Xinhua Hospital (No.12YJ01), the National Natural Science Foundation of China (No. 81172029, 81272402), the Foundation of Shanghai Outstanding Academic Leaders (No.11XD1403800), the National High Technology Research and Development Program (863 Program; No.2012AA022606), and the Medical and Engineering Cross-Foundation of Shanghai Jiaotong University (No. YG2011ZD07).

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No conflict of interest to disclose.

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Authors and Affiliations

  1. Department of General Surgery, Xinhua hospital, School of Medicine, Shanghai Jiaotong University, Room 615, NO.8 Building, 1665 kongjiang road, Shanghai, 200092, People’s Republic of China

    Maolan Li, Jun Shen, Xiangsong Wu, Hao Weng, Qian Ding, Zhujun Tan, Jiasheng Mu, Jiahua Yang, Yijun Shu, Runfa Bao, Qichen Ding, Wenguang Wu, Yang Cao & Yingbin Liu

  2. Department of General Surgery, Shanxi Medical University Second Hospital, Taiyuan, People’s Republic of China

    Bingtai Zhang & Guofeng Gao

  3. Department of Oncological Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Hohehot, People’s Republic of China

    Rui Zhang

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  1. Maolan Li
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  2. Jun Shen
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  3. Xiangsong Wu
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  10. Jiasheng Mu
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  11. Jiahua Yang
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  13. Runfa Bao
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  14. Qichen Ding
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  15. Wenguang Wu
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  16. Yang Cao
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  17. Yingbin Liu
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Corresponding author

Correspondence to Yingbin Liu.

Additional information

Maolan Li, Jun Shen contributed equally to this work.

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Li, M., Shen, J., Wu, X. et al. Downregulated expression of hepatoma-derived growth factor (HDGF) reduces gallbladder cancer cell proliferation and invasion. Med Oncol 30, 587 (2013). https://doi.org/10.1007/s12032-013-0587-7

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  • DOI: https://doi.org/10.1007/s12032-013-0587-7

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