Skip to main content

Advertisement

SpringerLink
Log in

Metabonomic Study of Biochemical Changes in Human Hair of Heroin Abusers by Liquid Chromatography Coupled with Ion Trap-Time of Flight Mass Spectrometry

  • Published:
Journal of Molecular Neuroscience Aims and scope Submit manuscript

Abstract

Hair analysis is with the advantage of non-invasive collection and long surveillance window. The present study employed a sensitive and reliable liquid chromatography coupled with ion trap-time of flight mass spectrometry method to study the metabonomic characters in the hair of 58 heroin abusers and 72 non-heroin abusers. Results indicated that certain endogenous metabolites, such as sorbitol and cortisol, were accelerated, and the level of arachidonic acid, glutathione, linoleic acid, and myristic acid was decreased in hair of heroin abusers. The metabonomic study is helpful for further understanding of heroin addiction and clinical diagnosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Deevanhxaya P, Suzukia M, Maeshibua N, Lia H, Tanakab K, Hirosea S (2009) Simultaneous characterization of quaternary alkaloids, 8-oxoprotoberberine alkaloids, and a steroid compound in Coscinium fenestratum by liquid chromatography hybrid ion trap time-of-flight mass spectrometry. J Pharm Biomed Anal 50:413

    Article  Google Scholar 

  • Nicholson JK, Lindon JC (2008) Systems biology: metabonomics. Nature 455:1054

    Article  CAS  PubMed  Google Scholar 

  • Zhang AH, Sun H, Wu XH, Wang XJ (2012) Urine metabolomics. Clinica Chimica Acta. 414:65

    Article  CAS  Google Scholar 

  • Cooper GAA, Moeller M, Kronstrand R (2008) Current status of accreditation for drug testing in hair. Forensic Sci Int 176:9

    Article  PubMed  Google Scholar 

  • Cooper GAA, Kronstrand R, Kintz P (2012) Society of Hair Testing guidelines for drug testing in hair. Forensic Sci Int 218:20

    Article  CAS  PubMed  Google Scholar 

  • M. J. Kreek, L. Borg, Y. Zhou, I. Kravets (2009) Hormones, brain and behavior (second edition). 3177

  • Zhu KY, Leung KW, Ting AKL, Wong ZCF, Fu Q, Ng WYY, Choi RCY, Dong TTX, Wang TJ, Lau DTW, Tsim KWK (2011) The establishment of a highly sensitive method in detecting ketamine and norketamine simultaneously in human hairs by HPLC-Chip-MS/MS. Forensic Sci Int 208:53

    Article  CAS  PubMed  Google Scholar 

  • Tsutsui H, Maeda T, Min JZ, Inagako S, Higashi T, Kagawa Y, Toyo’oka T (2011) Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry. Clinica Chimica Acta. 412:861

    Article  CAS  Google Scholar 

  • Zhang FX, Jia ZH, Gao P, Kong HW, Li X, Chen J, Yang Q, Yin PY, Wang JS, Lu X, Li FM, Wu YL, Xu GW (2009) Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry. Talanta 79:836

    Article  CAS  PubMed  Google Scholar 

  • Lee S, Cordero R, Paterson S (2009) Distribution of 6-monoacetylmorphine and morphine in head and pubic hair from heroin-related deaths. Forensic Sci Int 183:74

    Article  CAS  PubMed  Google Scholar 

  • Henderson GL (1993) Mechanisms of drug incorporation into hair. Forensic Sci Int 63:19

    Article  CAS  PubMed  Google Scholar 

  • Zhu KY, Leung KW, Ting AKL, Wong ZCF, Ng WYY, Choi RCY, Dong TTX, Wang TJ, Lau DTW, Tsim KWK (2012) Microfluidic chip based nano liquid chromatography coupled to tandem mass spectrometry for the determination of abused drugs and metabolites in human hair. Anal Bioanal Chem 402:2805

    Article  CAS  PubMed  Google Scholar 

  • Domínguez-Romero JC, García-Reyes JF, M-Díaz A (2011) Screening and quantitation of multiclass drugs of abuse and pharmaceuticals in hair by fast liquid chromatography electrospray time-of-flight mass spectrometry. J Chromatogr B 879:2034

    Article  Google Scholar 

  • Salomone A, Gerace E, Brizio P, Gennaro MC, Vincenti M (2011) A fast liquid chromatography-tandem mass spectrometry method for determining benzodiazepines and analogues in urine. Validation and application to real cases of forensic interest. J Pharm Biomed Anal 56:582

    Article  CAS  PubMed  Google Scholar 

  • Höld KM, Wilkins DG, Rollins DE, Joseph RE, Cone EJ (1998) Simultaneous quantitation of cocaine, opiates, and their metabolites in human hair by positive ion chemical ionization gas chromatography-mass spectrometry. J Chromatogr Sci 36:125

    Article  PubMed  Google Scholar 

  • Ballabio D, Consonni V (2013) Classification tools in chemistry. Part 1: linear models. PLS-DA. Analytical methods. Anal. Methods 5:3790

    Article  CAS  Google Scholar 

  • Stalder T, Kirschbaum C (2012) Analysis of cortisol in hair–state of the art and future directions. Brain, Behavior, and Immunity 26:1019

    Article  CAS  PubMed  Google Scholar 

  • Ashwell JD, Lu FWM, Vacchio MS (2000) Glucocorticoids in T cell development and function. Annu Rev Immunol 18:309

    Article  CAS  PubMed  Google Scholar 

  • Chrousos GP (2009) Stress and disorders of the stress system. Endocrinol Nat Rev Endocrinol 5:374

    Article  CAS  PubMed  Google Scholar 

  • Price RK, Welch RW (2013) pp. 209–214 Cereal grains. In: In: encyclopedia of Human Nutrition, 3rd edn. Academic Press, Waltham, MA

    Google Scholar 

  • Choque B, Catheline D, Rioux V, Legrand P (2014) Linoleic acid: between doubts and certainties. Biochimie Lipids Metab Diseases 96:14

    CAS  Google Scholar 

  • Fukamachi N, Watanabe Y, Kobayashi B (1988) Inhibition of thrombin-induced platelet aggregation by myristic acid. Thrombosis Research. 52:227

    Article  CAS  PubMed  Google Scholar 

  • Beauchamp E, Goenaga D, Le Bloc'h J, Catheline D, Legrand P, Rioux V (2007) Myristic acid increases the activity of dihydroceramide delta-4-desaturase 1 through its N-terminal myristoylation. Biochimie 89:1553

    Article  CAS  PubMed  Google Scholar 

  • Zock PL, de Vries JH, Katan MB (1994) Impact of myristic acid versus palmitic acid on serum lipid and lipoprotein levels in healthy women and men. Arteriosclerosis, Thrombosis, and Vascular Biology 14:567

    Article  CAS  Google Scholar 

  • Rioux V, Pédrono F, Legrand P (2011) Regulation of mammalian desaturases by myristic acid: N-terminal myristoylation and other modulations. Biochimica Biophysica Acta. 181:1

    Article  Google Scholar 

  • Martin DDO, Beauchamp E, Berthiaume LG (2011) Post-translational myristoylation: fat matters in cellular life and death. Biochimie 93:18

    Article  CAS  PubMed  Google Scholar 

  • Zheng T, Liu LS, Aa JY, Wang GJ, Cao B, Li MJ, Shi J, Wang XW, Zhao CY, Gua RR, Zhou J, Xiao WJ, Yua XY, Sun RB, Zhou YH, Zuo YX, Zhu XX (2013) Metabolic phenotype of rats exposed to heroin and potential markers of heroin abuse. Drug and Alcohol Dependence. 127:177

    Article  CAS  PubMed  Google Scholar 

  • Vuong C, Van Uum SH, O'Dell LE, Lutfy K, Friedman TC (2010) The effects of opioids and opioid analogs on animal and human endocrine systems. Endocrinol. Rev. 31:98

    Article  CAS  Google Scholar 

  • Gaynes BI, Watkins III JB (1989) Comparison of glucose, sorbitol and fructose accumulation in lens and liver of diabetic and insulin-treated rats and mice. Comp Biochem Physiol Part B: Comp Biochem 92:685

    Article  CAS  Google Scholar 

  • Koyama AH, Arakawa T, Adachi A (2000) Characterization of apoptosis induced by sorbitol: a unique system for the detection of antiapoptotic activities of viruses. Microbes Infect 2:599

    Article  CAS  PubMed  Google Scholar 

  • Harrell PT, Mancha BE, Petras H, Trenz RC, Latimer WW (2012) Latent classes of heroin and cocaine users predict unique HIV/HCV risk factors. Drug and Alcohol Dependence 122:220

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kidd PM (1997) Systemic protectant against oxidative and free radical damage. Altern Med Rev 2:155

    Google Scholar 

  • Li ZH, Liu YF, Li KN, DuanMu HZ, Chang ZQ, Li ZQ, Zhang SZ, Xu Y (2012) Analysis of functional and pathway association of differential co-expressed genes: a case study in drug addiction. J. Biomed. Informatics. 45:30

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by Shenzhen and Hong Kong Innovative Circle Project (No. JSE201007160003A). The authors would like to thank Mr. Xin-jun Sun in Shenzhen Detoxification Center of Public Security Bureau for the collection of hair from heroin users.

Conflict of Interest

The authors declare that they have no competing interests.

Compliance with Ethical Standards

This research has been approved by the ethical committee of Shenzhen detoxification center of public security bureau (SZ2012-H092), and the written informed consent has been obtained from all the hair donors.

Author information

Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, People’s Republic of China

    Pu Xie, Qing Li & Kai-shun Bi

  2. Shenzhen Institute for Drug Control, Shenzhen, 518057, People’s Republic of China

    Pu Xie, Tie-jie Wang, Guo Yin, Yan Yan & Li-he Xiao

Authors
  1. Pu Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tie-jie Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guo Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li-he Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kai-shun Bi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kai-shun Bi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xie, P., Wang, Tj., Yin, G. et al. Metabonomic Study of Biochemical Changes in Human Hair of Heroin Abusers by Liquid Chromatography Coupled with Ion Trap-Time of Flight Mass Spectrometry. J Mol Neurosci 58, 93–101 (2016). https://doi.org/10.1007/s12031-015-0655-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12031-015-0655-x

Keywords

Search

Navigation