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Microfluidic chip based nano liquid chromatography coupled to tandem mass spectrometry for the determination of abused drugs and metabolites in human hair

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Abstract

A microfluidic chip based nano-HPLC coupled to tandem mass spectrometry (nano-HPLC-Chip-MS/MS) has been developed for simultaneous measurement of abused drugs and metabolites: cocaine, benzoylecgonine, cocaethylene, norcocaine, morphine, codeine, 6-acetylmorphine, phencyclidine, amphetamine, methamphetamine, MDMA, MDA, MDEA, and methadone in the hair of drug abusers. The microfluidic chip was fabricated by laminating polyimide films and it integrated an enrichment column, an analytical column and a nanospray tip. Drugs were extracted from hairs by sonication, and the chromatographic separation was achieved in 15 min. The drug identification and quantification criteria were fulfilled by the triple quardropule tandem mass spectrometry. The linear regression analysis was calibrated by deuterated internal standards with all of the R 2 at least over 0.993. The limit of detection (LOD) and the limit of quantification (LOQ) were from 0.1 to 0.75 and 0.2 to 1.25 pg/mg, respectively. The validation parameters including selectivity, accuracy, precision, stability, and matrix effect were also evaluated here. In conclusion, the developed sample preparation method coupled with the nano-HPLC-Chip-MS/MS method was able to reveal the presence of drugs in hairs from the drug abusers, with the enhanced sensitivity, compared with the conventional HPLC-MS/MS.

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Acknowledgments

This research was supported by grants from the Office of the Vice-President for Research and Graduate Studies (VPRD011SC01), Croucher Foundation (CAS-CF07/08.SC03), Beat Drug Fund (BDF101014), and ITC (GHP/018/10SZ) awarded to KT.

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Authors and Affiliations

  1. Division of Life Science, and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong

    Kevin Y. Zhu, K. Wing Leung, Annie K. L. Ting, Zack C. F. Wong, Winki Y. Y. Ng, Roy C. Y. Choi, Tina T. X. Dong, Tiejie Wang, David T. W. Lau & Karl W. K. Tsim

  2. Shenzhen Institute for Drug Control, Shenzhen, 518029, China

    Tiejie Wang

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  1. Kevin Y. Zhu
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  2. K. Wing Leung
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  3. Annie K. L. Ting
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  4. Zack C. F. Wong
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  5. Winki Y. Y. Ng
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  10. Karl W. K. Tsim
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Corresponding author

Correspondence to Karl W. K. Tsim.

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Zhu, K.Y., Leung, K.W., Ting, A.K.L. et al. Microfluidic chip based nano liquid chromatography coupled to tandem mass spectrometry for the determination of abused drugs and metabolites in human hair. Anal Bioanal Chem 402, 2805–2815 (2012). https://doi.org/10.1007/s00216-012-5711-6

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  • DOI: https://doi.org/10.1007/s00216-012-5711-6

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