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Upregulated Expression of NF-YC Contributes to Neuronal Apoptosis via Proapoptotic Protein Bim in Rats’ Brain Hippocampus Following Middle Cerebral Artery Occlusion (MCAO)

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Abstract

Cerebral ischemia represents a severe brain injury that could lead to significant neuronal damage and death. In this study, we performed a middle cerebral artery occlusion (MCAO) in adult rats and observed that a subunit of nuclear factor-Y (NF-Y) transcriptional factor, NF-YC, was accumulated in rat hippocampal CA1 neurons. Immunochemistrical and immunofluorescent analysis revealed that NF-YC was primarily expressed in the nucleus of neurons. Meanwhile, we found that the changes of bim, one of the target genes of NF-Y, were consistent with the expression of NF-YC and Bim was mainly located in the NF-YC positive cells. Moreover, there was a concomitant upregulation of active caspase-3 and TUNEL positive cells. Taken together, these results suggested that the upregulation of NF-YC might play an important role in the pathophysiology via proapoptotic protein Bim after MCAO and further research is needed to have a better understanding of its function and mechanism.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81300720).

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Authors and Affiliations

  1. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, People’s Republic of China

    Yunfeng Wang, Lixiang Yang, Bing Li, Ting Lu, Yongfeng Bi & Gang Cui

  2. School of Public Health, Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Chunhua Wan & Junkang Jiang

  3. Department of Dermatology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, People’s Republic of China

    Shanshan Yu

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  1. Yunfeng Wang
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  2. Chunhua Wan
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  4. Lixiang Yang
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Correspondence to Junkang Jiang or Gang Cui.

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Yunfeng Wang, Chunhua Wan, Junkang Jiang, and Gang Cui have contributed equally to this work.

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Wang, Y., Wan, C., Yu, S. et al. Upregulated Expression of NF-YC Contributes to Neuronal Apoptosis via Proapoptotic Protein Bim in Rats’ Brain Hippocampus Following Middle Cerebral Artery Occlusion (MCAO). J Mol Neurosci 52, 552–565 (2014). https://doi.org/10.1007/s12031-013-0111-8

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