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Effects of PACAP in UV-A Radiation-Induced Retinal Degeneration Models in Rats

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Abstract

The retina is constantly exposed to ultraviolet (UV) light with different wavelengths, which may lead to chronic UV-induced retinal injury. In our previous studies, we have shown the protective effects of pituitary adenylate cyclase activating polypeptide (PACAP) in toxic and ischemic retinal injuries. The aim of the present study was to investigate the effects of PACAP in UV-A-induced retinal lesion. We used diffuse UV-A radiation (315–400 nm) to induce acute retinal damage over a short period of exposure. Using standard histological (morphological and morphometrical) analysis, we assessed the actions of intravitreal PACAP (100 pmol/5 µl) treatment on acute UV-A-induced retinal damage. We measured the thickness of nuclear and plexiform layers as well as the number of cells in the outer nuclear and inner nuclear layers and in the ganglion cell layer. Outer limiting membrane–inner limiting membrane distances in the cross-section of the retina were also examined. Our results show that UV-A light-induced retinal damage led to severe degeneration in the photoreceptor layer, and in the outer and inner nuclear layers. Alteration in the plexiform layers was also observed. We found that post-irradiation PACAP treatment significantly attenuated the UV-A-induced retinal damage. Our results provide the basis for future clinical application of PACAP treatment in retinal degeneration and may have clinical implications in several ophthalmic diseases.

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Acknowledgments

Hungarian National Scientific Grants OTKA T061766, K72592, F67830, CNK 78480, ETT278-04/2009, Richter Gedeon Centenary Foundation, Bolyai Scholarship, University of Pecs Medical School Research Grant 2009.

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Authors and Affiliations

  1. Department of Sportbiology, University of Pecs, Pecs, Hungary

    Tamas Atlasz

  2. Experimental Zoology and Neurobiology, Faculty of Sciences, Institute of Biology, University of Pecs, Pecs, Hungary

    Krisztina Szabadfi, Monika Griecs, Levente Hamza & Robert Gabriel

  3. Department of Anatomy, Medical School, University of Pecs, Pecs, Hungary

    Peter Kiss, Andrea Tamas & Dora Reglodi

  4. Department of General Physics and Laser Spectroscopy, Institute of Physics, University of Pecs, Pecs, Hungary

    Zsuzsanna Marton

  5. Department of Ophthalmology, University of Pecs, Pecs, Hungary

    Valeria Gaal & Zsolt Biro

  6. Department of Biophysics, Medical School, University of Pecs, Pecs, Hungary

    Gabor Hild & Miklos Nyitrai

  7. Department of Medical Chemistry, University of Szeged, Szeged, Hungary

    Gabor Toth

  8. Department of Anatomy, University of Pecs, Szigeti u 12, 7624, Pecs, Hungary

    Dora Reglodi

Authors
  1. Tamas Atlasz
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  2. Krisztina Szabadfi
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  3. Peter Kiss
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  4. Zsuzsanna Marton
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  5. Monika Griecs
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  6. Levente Hamza
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  7. Valeria Gaal
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  8. Zsolt Biro
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  9. Andrea Tamas
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  10. Gabor Hild
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  11. Miklos Nyitrai
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  12. Gabor Toth
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  13. Dora Reglodi
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  14. Robert Gabriel
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Corresponding author

Correspondence to Dora Reglodi.

Additional information

T. Atlasz, K. Szabadfi, D. Reglodi, and R. Gabriel contributed equally to the present work.

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Atlasz, T., Szabadfi, K., Kiss, P. et al. Effects of PACAP in UV-A Radiation-Induced Retinal Degeneration Models in Rats. J Mol Neurosci 43, 51–57 (2011). https://doi.org/10.1007/s12031-010-9392-3

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  • DOI: https://doi.org/10.1007/s12031-010-9392-3

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