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Imbalanced expression of Bcl-xL and Bax in platelets treated with plasma from immune thrombocytopenia

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Abstract

Immune thrombocytopenia is a heterogeneous autoimmune disease, characterized by accelerated platelet destruction and impaired platelet production. Bcl-xL and Bax play an opposite role in the regulation of apoptotic process with Bcl-xL for cell survival and Bax for cell apoptosis. Given the critical roles in the regulation of platelet apoptosis, whether Bcl-xL or Bax was involved in the pathogenesis of ITP remains unknown. The aim of this study is to evaluate the expression profile of Bcl-xL and Bax in platelets treated with ITP plasma. Normal washed platelets were treated with plasma from 20 active ITP patients or 10 age and gender-matched control to mimic the ITP in vivo environment. Mitochondrial depolarization, platelet apoptosis and activation were measured by flow cytometry. Expression of Bcl-xL, Bax and caspase-3 were also measured by quantitative real-time PCR and western blot. Our results demonstrated increased mitochondrial depolarization, platelet apoptosis and activation in platelets after treated with ITP plasma in comparison to control. In addition, decreased expression of Bcl-xL, increased expression of Bax and activity of caspase-3 were also observed. Furthermore, a negative correlation of Bcl-xL with Bax was found in platelets treated with ITP plasma. In conclusion, imbalanced expression of Bcl-xL and Bax might be associated with platelet apoptosis in ITP and therapeutically targeting them might be a novel approach in the treatment of ITP.

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Acknowledgments

This research was supported by National Natural Science Foundation of China (Grant Nos. 81400082 and 81370602), the Natural Science Foundation of Jiangsu Province (Grant Nos. BK20140219 and BK2012573) and China Postdoctoral Science Foundation funded project (Project No. 2015M570479).

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    Authors and Affiliations

    1. Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, 99, West Huaihai Rd, Quanshan District, Xuzhou, 221002, Jiangsu, China

      Jianlin Qiao, Depeng Li, Yao Yao, Mingshan Niu, Chunling Fu, Zhenyu Li, Kailin Xu & Lingyu Zeng

    2. Blood Diseases Institute, Xuzhou Medical College, Xuzhou, 221002, China

      Jianlin Qiao, Yulu Wu, Xiaoqian Li, Yao Yao, Mingshan Niu, Chunling Fu, Kailin Xu & Lingyu Zeng

    3. Key Laboratory of Bone Marrow Stem Cell, Xuzhou, 221002, Jiangsu Province, China

      Jianlin Qiao, Yulu Wu, Xiaoqian Li, Yao Yao, Mingshan Niu, Chunling Fu, Kailin Xu & Lingyu Zeng

    4. Department of Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China

      Yun Liu, Hongchun Li & Ping Ma

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    1. Jianlin Qiao
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    2. Yun Liu
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    Correspondence to Kailin Xu or Lingyu Zeng.

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    Jianlin Qiao and Yun Liu have contributed equally to this study.

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    Qiao, J., Liu, Y., Li, D. et al. Imbalanced expression of Bcl-xL and Bax in platelets treated with plasma from immune thrombocytopenia. Immunol Res 64, 604–609 (2016). https://doi.org/10.1007/s12026-015-8760-z

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    • DOI: https://doi.org/10.1007/s12026-015-8760-z

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