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CircMYC Regulates Glycolysis and Cell Proliferation in Melanoma

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Abstract

Circular RNAs (cicRNAs) have been identified to play pivotal roles in several cancer types. However, functions of circRNA in malignant melanoma are poor defined. Our current study demonstrated that human circMYC was obviously upregulated in human melanoma tissue. Furthermore, circMYC promoted the proliferation of human melanoma cells and Mel-CV cells. The expression of circMYC can repress Mel-CV cell glycolysis and LDHA activities in the in vitro glycolysis and lactate production evaluations. circMYC directly bound to miR-1236 as a molecular sponge that targeting miR-1236 in Mel-CV cells via bioinformatics analysis, pull-down assay, and luciferase reporter assays. Our present study revealed that 3′ UTR of LDHA acted as a target of miR-1236 using Mel-CV cells. Based on our findings, c-MYC-SRSF1 axis may regulate the production of circMYC. Overall, these results elucidate potential effects of circMYC in melanoma development and provide a promising biomarker for melanoma diagnosis.

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Funding

The study was supported by the Wuxi municipal health and Family Planning Commission Fund (MS201711).

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  1. These authors contributed equally: Cheng Jin, Dake Dong

Authors and Affiliations

  1. Department of Dermatology, The Affiliated Wuxi No. 2 People’s Hospital of Nanjing Medical University, No. 68 Zhongshan Road, Wuxi, 214002, Jiangsu, China

    Cheng Jin, Rushan Xia, Shiqin Tao & Meishan Piao

  2. Department of Dermatology, The Affiliated Hospital of Jiangnan University, No. 200 Huihe Road, Wuxi, 214062, Jiangsu, China

    Dake Dong

  3. MBA Education Center, School of Business Jiangnan University, No. 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China

    Zhen Yang

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Jin, C., Dong, D., Yang, Z. et al. CircMYC Regulates Glycolysis and Cell Proliferation in Melanoma. Cell Biochem Biophys 78, 77–88 (2020). https://doi.org/10.1007/s12013-019-00895-0

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