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Biocompatibility of Oxygen-Sensing Paramagnetic Implants

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Abstract

Oxygen-sensing implants, composed of paramagnetic microcrystals embedded in a biocompatible polymer, are increasingly being used for electron paramagnetic resonance (EPR) oximetry in animal models and human subjects. The implants are stable and designed to stay in the tissues for indefinite periods. However, it is not known whether the crystals that may be exposed on the surface of the implants or leached out from the implants will induce cytotoxicity thereby compromising their biocompatibility over the long term. The goal of the current study was to evaluate the cytotoxicity of the implants and crystalline particulates under in vitro conditions. Apoptosis and cell viability studies were performed using L6, a rat muscle cell line and AsPC-1, a cancer cell line derived from human pancreatic adenocarcinoma. The results indicated that neither the intact implants nor their components elicit cytotoxicity, thus establishing their biocompatibility for use in human subjects.

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Acknowledgements

This study was supported by the National Institutes of Health grants R01 EB004031 and P01 CA190193.

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Authors and Affiliations

  1. Department of Radiology, Geisel School of Medicine, Dartmouth College, 1 Medical Center Drive, Lebanon, NH, 03756, USA

    Dan Tse & Periannan Kuppusamy

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  1. Dan Tse
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  2. Periannan Kuppusamy
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Correspondence to Periannan Kuppusamy.

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Tse, D., Kuppusamy, P. Biocompatibility of Oxygen-Sensing Paramagnetic Implants. Cell Biochem Biophys 77, 197–202 (2019). https://doi.org/10.1007/s12013-019-00881-6

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  • DOI: https://doi.org/10.1007/s12013-019-00881-6

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