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Evaluation of Angiogenesis in Non-small Cell Lung Carcinoma by CD34 Immunohistochemistry

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Abstract

The objective of the study was to investigate angiogenesis in non-small lung cancer by measuring the expression of CD34. Immunohistochemistry was used to detect CD34 at the endothelial cell surface in 81 surgically resected non-small cell lung cancer specimens. CD34 immunohistochemistry had high specificity and sensitivity with minimal background, which enabled efficient identification of CD34-positive staining. Statistical analysis showed that increased microvessel density (MVD) was closely correlated to tumor progression as reflected by the clinical stage (P < 0.05). However, MVD was not significantly associated with lymph node metastasis and other clinical and pathological features (P > 0.05). In conclusion, microvascular density may play an important role in the development and progression of lung cancer.

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Acknowledgments

This project was funded by the project Innovation of Science and Technology, Chongqing Science and Technology Commission (project numbers: 98028 and cstc2013jcyjA10108); and the Foundation of Bureau of Public Health of Chongqing (project numbers: 06-2-001 and 2010-2-127).

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Authors and Affiliations

  1. Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Linjiang Road 74, Yuzhong District, Chongqing, 400010, China

    Zhu Bing, Jiang Yao-quan & Chen Shi-feng

  2. Central Laboratory, No. 6 Hospital of Handan City, Handan, 056000, China

    Yang Jian-ru

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  1. Zhu Bing
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  2. Yang Jian-ru
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  3. Jiang Yao-quan
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  4. Chen Shi-feng
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Correspondence to Zhu Bing.

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Bing, Z., Jian-ru, Y., Yao-quan, J. et al. Evaluation of Angiogenesis in Non-small Cell Lung Carcinoma by CD34 Immunohistochemistry. Cell Biochem Biophys 70, 327–331 (2014). https://doi.org/10.1007/s12013-014-9916-5

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  • DOI: https://doi.org/10.1007/s12013-014-9916-5

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