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Bone Morphogenetic Protein-2-Encapsulated Grafted-Poly-Lactic Acid–Polycaprolactone Nanoparticles Promote Bone Repair

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Abstract

The aim of this study is to test the efficacy of a novel tissue-engineered bone in repairing bone defects, using poly-lactic-acid–polycaprolactone (PLA–PCL) scaffolding seeded with PEG-bone morphogenetic protein-2 (BMP-2)-transfected rBMSCs (rabbit bone marrow stromal cells). The rBMSCs were transfected with PEG/BMP-2 or liposome/BMP-2, and then implanted into a PLA–PCL tissue-engineered bone. The protein level of BMP-2 was assessed by Western blot analysis and immunohistochemistry. ELISA was used to measure the amount of BMP-2 secreted in the culture media. The mRNA level of BMP-2 and osteocalcin was assayed quantitatively by real-time PCR. The middle portion of the bilateral radius in New Zealand rabbits was excised and implanted with tissue-engineered bone, and the modified areas were monitored by X-ray, hematoxylin–eosin staining, and immunohistochemistry staining of BMP-2. PEG–BMP-2 nanoparticles (NPs) and BMP-2-loaded PEG–PLA–PCL tissue-engineered bones were successfully constructed. The novel PEG–PLA–PCL NPs/DNA complex was a superior option for transfecting BMP-2 in rBMSCs compared to normal liposomes Moreover, the mRNA level of osteocalcin and alkaline phosphatase activity was also elevated upon transfection of BMP-2-encapsulated NPs. In vivo implants with BMP-2-carried tissue-engineered bone exhibited dramatic augmentation of BMP-2 and effective bone formation in the rabbit ectopic model. The PEG–PLA–PCL NPs/BMP-2 complex had an advantageous effect on bone repair, which provided an important theoretic basis for potential clinical treatments.

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Acknowledgments

This study was supported by Science and Technology Research Project of Liaoning Provincial Department of Education, No. 2010652.

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The authors have not declared any conflicts of interest.

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Authors and Affiliations

  1. Department of Traumatic Orthopaedic, Shengjing Hospital of China Medical University, 36 Sanhao Road, Heping District, Shenyang, 110004, Liaoning Province, China

    Xiaojun Xu, Jun Yang, Lifeng Ding & Jianjun Li

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  1. Xiaojun Xu
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  2. Jun Yang
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  3. Lifeng Ding
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  4. Jianjun Li
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Correspondence to Xiaojun Xu.

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Xu, X., Yang, J., Ding, L. et al. Bone Morphogenetic Protein-2-Encapsulated Grafted-Poly-Lactic Acid–Polycaprolactone Nanoparticles Promote Bone Repair. Cell Biochem Biophys 71, 215–225 (2015). https://doi.org/10.1007/s12013-014-0187-y

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