Abstract
Bone reconstruction is essential in orthodontic treatment that caters to the correction of malocclusion by bone reconstruction. Mesenchymal stem cells (MSCs) have been demonstrated a great potency of osteogenesis. The aim of this study was to investigate the effect of hypoxia on the rat bone marrow MSCs (rBMSCs) in vitro during osteogenesis. In this study, we found that temporary exposure of rBMSCs after osteogenic induction for 7 days to hypoxia (2% oxygen) led to a marked decrease in ALPase activity and the expression of osteocalcin and Runt related transcription factor 2/core binding factor a1 (Runx2/Cbfa1). Meanwhile, we found that exposure to hypoxia led to an early and transient increase in the level of phosphorylated ERK1/2 but had no obvious effects on mitogen-activated protein kinase (p38 MAPK) level. Based on these results, we concluded that hypoxia could inhibit osteogenic differentiation of rBMSCs possibly through MEK-ERK 1/2, while p38 MAPK may not participate in this regulation. Further exploration into the mechanisms of hypoxia on osteogenesis would surely provide reliable evidence for clinical practice.
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Abbreviations
- ALPase:
-
Alkaline phosphatase
- OC:
-
Osteocalcin
- rBMSCs:
-
Rat bone marrow mesenchymal stem cells
- VEGF:
-
Vascular endothelial growth factor
- Runx2:
-
Runt related transcription factor 2
- Cbfa1:
-
Core binding factor a1
- hMSCs:
-
Human mesenchymal stem cells
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Acknowledgments
We greatly thank Zhiguang Su for carefully reading the manuscript. This work was supported by grants from the National Natural Science Foundation of China [No. 30870597, 30901698, and 30900286].
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Yating Wang and Juan Li have contributed equally to this work.
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Wang, Y., Li, J., Wang, Y. et al. Effects of hypoxia on osteogenic differentiation of rat bone marrow mesenchymal stem cells. Mol Cell Biochem 362, 25–33 (2012). https://doi.org/10.1007/s11010-011-1124-7
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DOI: https://doi.org/10.1007/s11010-011-1124-7