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NF-κB p65 Subunit DNA-Binding Activity: Association with Depleted Antioxidant Levels in Breast Carcinoma Patients

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Abstract

NF-κB is recognized as a redox-sensitive transcription factor and has been implicated in cellular response to oxidative stress. The study was designed to correlate the changes in antioxidant status with the levels of nuclear factor-κB (NF-κB) p65 subunit DNA-binding activity in relation to lymph node involvement, tumor size, and staging in breast carcinoma patients. Case control study comprised of 40 breast carcinoma patients along with 40 age- and sex-matched healthy subjects as controls. Levels of enzymatic/nonenzymatic antioxidants along with the trace elements were measured to study the antioxidant status in the study subjects. Levels of NF-κB p65 subunit DNA-binding activity was estimated by ELISA assay. The levels of enzymatic, nonenzymatic antioxidants, and trace elements were found to be significantly depleted in breast carcinoma patients in comparison to healthy controls suggesting significantly decreased levels of antioxidant activity in the breast carcinoma patients. Also, these results indicate that antioxidant levels decrease progressively with the advancement of stage and subsequent progression of disease. DNA-binding activity of NF-κB p65 subunit was higher in breast cancer patients in comparison to normal healthy controls, and the activity was found to increase with the advancement of disease. Significant correlation was observed between the DNA-binding activity of NF-κB p65 subunit and antioxidant status in the patients. The logistic regression analysis revealed decreased levels of antioxidants and increased level of DNA-binding activity of NF-κB p65 subunit were significantly associated with incidence of breast carcinoma. Depleted antioxidant status and increased level of DNA-binding activity of NF-κB p65 subunit thus point clearly of an association in relation to disease progression, clinical stage, and cytological grade in the pathophysiology of breast carcinoma.

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Acknowledgments

This study was supported by Banaras Hindu University, Varanasi (India). The authors would like to thank the Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi (India) for providing blood samples for this study.

Conflict of interest

None of the authors has any financial or personal relationship with the organizations which could potentially be perceived as influencing the described research. All the authors have read the journal’s policy on disclosure of potential conflicts of interest.

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Authors and Affiliations

  1. Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Deepti Pande, Kanchan Karki, Reena Negi & H. D. Khanna

  2. Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Seema Khanna

  3. Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Ranjana S. Khanna

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  1. Deepti Pande
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  2. Kanchan Karki
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  3. Reena Negi
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  4. Seema Khanna
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  5. Ranjana S. Khanna
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  6. H. D. Khanna
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Correspondence to H. D. Khanna.

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Pande, D., Karki, K., Negi, R. et al. NF-κB p65 Subunit DNA-Binding Activity: Association with Depleted Antioxidant Levels in Breast Carcinoma Patients. Cell Biochem Biophys 67, 1275–1281 (2013). https://doi.org/10.1007/s12013-013-9645-1

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  • DOI: https://doi.org/10.1007/s12013-013-9645-1

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