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In Vivo and In Vitro Cardioprotective Effect of Gossypin Against Isoproterenol-Induced Myocardial Infarction Injury

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Abstract

The aim of the study was to examine the protective effects and possible mechanism of gossypin against isoproterenol (ISO)-mediated myocardial damage in vivo and H9c2 cell damage in vitro. H9c2 cells were categorized into five groups. Viability was evaluated with MTT and LDH release in H9c2 cells. Apoptotic parameter analysis was performed with cytochrome c (Cyt-c), caspase-3 (CASP-3), and BCL2/Bax mRNA expression levels. In vivo, gossypin was administered orally to mice at doses of 5, 10, and 20 mg/kg for 7 days. ISO groups were injected with isoproterenol (150 mg/kg) subcutaneously (on 8th and 9th) for 2 days. Afterward, lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB) levels and Troponin-I (Tn-I) amount from their serum, oxidative stress parameters superoxide dismutase (SOD) activity, glutathione (GSH) and malondialdehyde (MDA) levels, and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1 β), and NF-kB mRNA expression levels with inflammatory markers from heart tissue were evaluated. In addition, IL-1B, BCL-2, and cas-3 immunohistochemical staining was performed from heart tissue and TNF-a level was measured by ELISA method. Administration of Gossypin protected the cells by dose-dependent, eliminating the reduced cell viability and increased LDH release of ISO in H9c2 cells. In mice serum analyses, increased LDH, CK-MB levels, and Tn-I levels were normalized by gossypin. ISO administration in heart tissue is regulated by gossypin with increased SOD activity, GSH amount, TNF-α, IL-6, IL-1β, and NF-kB mRNA expression levels and decreased MDA amount. Overall, the present results demonstrated that gossypin has a potential cardioprotective treatment for ischemic heart disease on in vivo and in vitro.

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Data Availability

Data that support the plots within this publication and other findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, Kastamonu University, 3700, Kastamonu, Turkey

    Irfan Cinar

  2. Faculty of Medicine, Department of Pharmacology, Kafkas University, Kars, Turkey

    Muhammed Yayla

  3. Faculty of Medicine, Department of Pharmacology, Atatürk University, Erzurum, Turkey

    Taha Tavaci & Hamza Halici

  4. Faculty of Medicine, Department of Histology and Embriology, Kafkas University, Kars, Turkey

    Erdem Toktay & Pınar Bayram

  5. Faculty of Pharmacy, Department of Pharmacology, Atatürk University, Erzurum, Turkey

    Rustem Anil Ugan

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  1. Irfan Cinar
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  2. Muhammed Yayla
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  3. Taha Tavaci
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Contributions

IC and MY conceptualized and designed the research. TT, ETPB, RAU, and HH performed most of the experiments and analyzed the data with the help from IC and MY. IC and MY wrote the manuscript.

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Correspondence to Irfan Cinar.

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Cinar, I., Yayla, M., Tavaci, T. et al. In Vivo and In Vitro Cardioprotective Effect of Gossypin Against Isoproterenol-Induced Myocardial Infarction Injury. Cardiovasc Toxicol 22, 52–62 (2022). https://doi.org/10.1007/s12012-021-09698-3

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