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Effects of Fluorine on Intestinal Structural Integrity and Microbiota Composition of Common Carp

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Abstract

Fluorine is an environmental toxicant and exposure of fluorine could induce various health disorders. Gut microbiota has been known to be involved in maintaining animal or human health. Therefore, in the present study, we aimed to evaluate the relationship between fluorine exposure and gut microbiota in common carp. Gut microbiota composition was detected by 16S rRNA gene sequencing. Intestinal structural integrity was assessed by hematoxylin-eosin staining and tight junction protection detection. The results showed that exposure of carp to fluorine led to the injury of intestinal tissues. And compared to the control group, the expression of tight junction protein ZO-1 and occludin was decreased. Meanwhile, the gut microbial diversity and composition were changed by fluorine exposure. At the phylum level, the abundance of Fusobacteria and Firmicutes increased significantly, and the abundance of Actinobacteria decreased markedly after treatment of fluorine. At the genus level, interestingly, we found the abundance of Plesiomonas, an important pathogenic bacteria, increased significantly by the treatment of fluorine. And the abundance of Akkermansia, a critical probiotics, was markedly inhibited by the treatment of fluorine. In conclusion, the results suggested fluorine exposure changed the gut microbiome composition and led to the damage of intestinal structural integrity.

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Funding

The work was supported by the National Natural Science Foundation of China (no. 30972191), Jilin Province Industrial Technology Research and Development Special Project (no. 2019C059-5), and Jilin Province Science and Technology Development Plan Project (no. 20190201179JC).

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Authors and Affiliations

  1. School of Public Health of Jilin University, Changchun, China

    Huiyuan Yu & Yuting Lu

  2. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Yue Zhang, Xinchi Shang & Yuehong Li

  3. Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun, 130118, China

    Yue Zhang, Xinchi Shang & Yuehong Li

  4. Health Monitoring and Inspection Center of Jilin Province, Changchun, 130062, China

    Peijun Zhang

  5. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, 130062, Jilin Province, People’s Republic of China

    Yuting Lu & Yunhe Fu

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  1. Huiyuan Yu
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Correspondence to Yunhe Fu or Yuehong Li.

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The experiments were according to the Chinese regulation for Experimental Animals and approved by the Institutional Animal Care and Use Committee of Jilin Agricultural University.

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Yu, H., Zhang, Y., Zhang, P. et al. Effects of Fluorine on Intestinal Structural Integrity and Microbiota Composition of Common Carp. Biol Trace Elem Res 199, 3489–3496 (2021). https://doi.org/10.1007/s12011-020-02456-6

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  • DOI: https://doi.org/10.1007/s12011-020-02456-6

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