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Analysis of Antioxidant and Anti-inflammatory Activity of Silicon in Murine Macrophages

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Abstract

The purpose of this study is to investigate the antioxidant and anti-inflammatory properties of silicon (Si) in the RAW 264.7 murine macrophage cell line. Lipopolysaccharide (LPS) was used to induce inflammatory conditions, and cells were treated with 0, 1, 5, 10, 25, 50, and 100 μM Si in the form of sodium metasilicate. Tert-butylhydroquinone (TBHQ), a well-known antioxidative substance, was used as a positive control to assess the degree of antioxidative and anti-inflammatory properties of Si. Sodium metasilicate at 100 μM suppressed LPS-induced nitric oxide generation from macrophages 36 h after treatment. In addition, 50 μM sodium metasilicate decreased interleukin-6 production, and the degree of suppression was comparable to that of 10 μM TBHQ treatment. LPS-induced messenger RNA (mRNA) expression of tumor necrosis factor-α and inducible nitric oxide synthase was significantly decreased by 1, 5, 10, and 50 μM sodium metasilicate. Cyclooxygenase-2 mRNA expression was also suppressed by 1, 5, 25, and 50 μM sodium metasilicate. Based on these data, Si has the ability to suppress the production of inflammatory cytokines and mediators, possibly through the suppression of radical scavenger activity and down-regulation of gene expression of inflammatory mediators.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (no. 2011-0010880).

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Authors and Affiliations

  1. Division of Food Science, Kongju National University, Yesan, 340-702, South Korea

    Eun-Jin Kim & Mi-Kyeong Choi

  2. Department of Food and Nutrition, Daegu University, Gyeongsan, 712-714, South Korea

    So-Young Bu

  3. Department of Food Science and Nutrition, Sookmyung Women’s University, Seoul, 140-742, South Korea

    Mi-Kyung Sung

  4. Department of Food and Nutrition, Hoseo University, Asan, 336-795, South Korea

    Myung-Hwa Kang

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  1. Eun-Jin Kim
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Correspondence to Mi-Kyeong Choi.

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Kim, EJ., Bu, SY., Sung, MK. et al. Analysis of Antioxidant and Anti-inflammatory Activity of Silicon in Murine Macrophages. Biol Trace Elem Res 156, 329–337 (2013). https://doi.org/10.1007/s12011-013-9829-y

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  • DOI: https://doi.org/10.1007/s12011-013-9829-y

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