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Effect of Boron on Osteogenic Differentiation of Human Bone Marrow Stromal Cells

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Abstract

Bone marrow stromal cells (BMSCs) have been well established as an ideal source of cell-based therapy for bone tissue engineering applications. Boron (B) is a notable trace element in humans; so far, the effects of boron on the osteogenic differentiation of BMSCs have not been reported. The aim of this study was to evaluate the effects of boron (0, 1, 10,100, and 1,000 ng/ml) on osteogenic differentiation of human BMSCs. In this study, BMSCs proliferation was analyzed by cell counting kit-8 (CCK8) assay, and cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay, Von Kossa staining, and real-time PCR. The results indicated that the proliferation of BMSCs was no different from the control group when added with B at the concentration of 1, 10, and 100 ng/ml respectively (P > 0.05); in contrast, 1,000 ng/ml B inhibited the proliferation of BMSCs at days 4, 7, and 14 (P < 0.05). By ALP staining, we discovered that BMSCs treated with 10 and 100 ng/ml B presented a higher ALP activity compared with control (P < 0.05). By real-time PCR, we detected the messenger RNA expression of ALP, osteocalcin, collagen type I, and bone morphogenetic proteins 7 were also increased in 10 and 100 ng/ml B treatment groups (P < 0.05). The calcium depositions were increased in 1 and 10 ng/ml B treatment groups (P < 0.05). Taken all together, it was the first time to report that B could increase osteogenic effect by stimulating osteogenic differentiation-related marker gene synthesis during the proliferation and differentiation phase in human BMSCs and could be a promising approach for enhancing osteogenic capacity of cell-based construction in bone tissue engineering.

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Acknowledgments

The authors thank all the staff in the Laboratory of Orthopaedic Research Institute and Scientific Research Center of Second Affiliated Hospital of Wenzhou Medical College. This work was supported by grants from the National Natural Science Foundation of China (30800220/C100201).

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, 109 Xue Yuan Xi Road, Wenzhou, 325000, China

    Xiaozhou Ying, Shaowen Cheng, Wei Wang, Qingyu Chen, Wei Zhang, Dongquan Kou, Yue Shen, Xiaojie Cheng & Lei Peng

  2. The Second Affiliated Hospital of Wenzhou Medical College, Trauma Center of the Affiliated Hospital of Hainan Medical College, 109 Xue Yuan Xi Road, Wenzhou, 325000, China

    Lei Peng

  3. Department of Orthopaedic Surgery, Hospital of Integrated Traditional Chinese and Western Medicine of Wenzhou, Wenzhou, China

    Zhongqin Lin

  4. Trauma Center of the Affiliated Hospital of Hainan Medical College, 31 Long Hua Road, Haikou, 571100, China

    Ferdinand An Rompis & Chuan zhu Lu

Authors
  1. Xiaozhou Ying
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  2. Shaowen Cheng
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  3. Wei Wang
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  4. Zhongqin Lin
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  5. Qingyu Chen
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  6. Wei Zhang
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  7. Dongquan Kou
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  8. Yue Shen
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  9. Xiaojie Cheng
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  10. Ferdinand An Rompis
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  11. Lei Peng
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  12. Chuan zhu Lu
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Corresponding authors

Correspondence to Lei Peng or Chuan zhu Lu.

Additional information

X. Ying and S. Cheng contributed equally to this work.

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Ying, X., Cheng, S., Wang, W. et al. Effect of Boron on Osteogenic Differentiation of Human Bone Marrow Stromal Cells. Biol Trace Elem Res 144, 306–315 (2011). https://doi.org/10.1007/s12011-011-9094-x

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  • DOI: https://doi.org/10.1007/s12011-011-9094-x

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