Abstract
Adequate thyroid hormone is critical for cerebellar development. Developmental hypothyroidism induced by iodine deficiency during gestation and postnatal period results in permanent impairments of cerebellar development with an unclear mechanism. In the present study, we implicate cerebellar caveolin-1 and synaptotagmin-1, the two important molecules involved in neuronal development, in developmental iodine deficiency, and in developmental hypothyroidism. Two developmental rat models were created by administrating dam rats with either iodine-deficient diet or propylthiouracil (PTU, 5 or 15 ppm)-added drinking water from gestational day 6 till postnatal day (PN) 28. Nissl staining and the levels of caveolin-1 and synaptotagmin-1 in cerebella were assessed on PN28 and PN42. The results show that the numbers of Purkinje cells were reduced in the iodine-deficient and PTU-treated rats. The upregulation of caveolin-1 and the downregulation of synaptotagmin-1 were observed in both iodine-deficient and PTU-treated rats. These findings may implicate decreases in the number of Purkinje cells and the alterations in the levels of caveolin-1 and synaptotagmin-1 in the impairments of cerebellar development induced by developmental iodine deficiency and hypothyroidism.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China [grant number 30800896] and also supported by Natural Science Foundation of Liaoning Province, China for Ph.D. holders [grant number 20081053]. The excellent theoretical and technical help of Professor Hong Lai and Professor Zhanyou Wang from the College of Basic Medical Sciences at China Medical University is gratefully acknowledged.
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Wang, Y., Zhong, J., Wei, W. et al. Developmental Iodine Deficiency and Hypothyroidism Impair Neural Development, Upregulate Caveolin-1, and Downregulate Synaptotagmin-1 in the Rat Cerebellum. Biol Trace Elem Res 144, 1039–1049 (2011). https://doi.org/10.1007/s12011-011-9089-7
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DOI: https://doi.org/10.1007/s12011-011-9089-7