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Effect of Magnesium Deficiency on Various Mineral Concentrations in Rat Liver

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Abstract

Magnesium (Mg) deficiency is well known to affect metabolism of some trace minerals. We investigated the effect of Mg deficiency on hepatic concentration of various minerals in rats. Twelve 5-week-old male rats were divided into the groups given a control diet and an Mg-deficient diet. After 4 weeks, liver sample was collected from each rat. The concentrations of 36 minerals were simultaneously determined by a semiquantitative method of inductively coupled plasma-mass spectrometry (ICP-MS). The semiquantitative analysis showed that Mg deficiency significantly increased iron (Fe), copper (Cu), zinc (Zn), gallium (Ga), yttrium (Y), zirconium (Zr), molybdenum (Mo), rhodium (Rh), silver (Ag), and barium (Ba) concentrations, and significantly decreased scandium (Sc) and niobium (Nb) concentrations in rat liver. Then, hepatic Fe, Cu, Zn, Sc, Zr, and Mo concentrations were quantitatively measured, which indicated the similar effects as observed by the semiquantitative analysis. Additionally, the semiquantitative measurements of these minerals were highly correlated to these measurements with the quantitative method, but the measurements were not completely consistent between these analyses. The present study is the first research indicating the changes of hepatic Ga, Y, Zr, Mo, Rh, Ag, Ba, Sc, and Nb concentrations in Mg-deficient rats. The present study also indicates that the semiquantitative analysis with ICP-MS is a valid method for screening analysis to investigate various mineral concentrations in animal tissues.

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Acknowledgments

The authors thank Emeritus Professor Yoshinori Itokawa and former Associate Professor Mieko Kimura of Division of Social and Preventive Medicine, Graduate School of Medicine, Kyoto University for giving us a chance to use ICP-MS.

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Authors and Affiliations

  1. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Ki Hyun Kim, Natsumi Ishizaki, Erika Iguchi, Masayuki Funaba & Tohru Matsui

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  1. Ki Hyun Kim
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  2. Natsumi Ishizaki
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  3. Erika Iguchi
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  4. Masayuki Funaba
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  5. Tohru Matsui
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Correspondence to Tohru Matsui.

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Kim, K.H., Ishizaki, N., Iguchi, E. et al. Effect of Magnesium Deficiency on Various Mineral Concentrations in Rat Liver. Biol Trace Elem Res 144, 865–871 (2011). https://doi.org/10.1007/s12011-011-9042-9

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  • DOI: https://doi.org/10.1007/s12011-011-9042-9

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