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Bioutilization of Chicken Feather Waste by Newly Isolated Keratinolytic Bacteria and Conversion into Protein Hydrolysates with Improved Functionalities

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Abstract

Chicken feathers are major poultry waste that is difficult to process in its native form due to highly resistant keratin protein in large amounts. In this study, a novel feather-degrading bacterium, Bacillus amyloliquefaciens KB1, was screened from a chicken farm bed (CFB) using morphological and biochemical tests followed by 16s rDNA analysis. Among observed isolates, bacterial isolate (KB1) showed the highest degree of feather degradation (74.78 ± 2.94%) and total soluble protein (205 ± 0.03 mg/g). The optimum fermentation conditions obtained were at 40 °C (temperature), pH 9, and 1% (w/v) feather concentration using response surface methodology in a Box-Behnken design. It produced 260 mg/g of soluble protein and bioactive peptides with 86.16% feather degradation. The amino acid profile showed an increase in the concentration of essential amino acids compared with the feather meal broth. The selection of a safe screening source for this new bacterium in CFB produced hydrolysates with enhanced bioactivity applicable for feed, and cosmetic applications, along with environmental bioremediation.

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Authors and Affiliations

  1. Food Engineering and Bioprocess Technology Program, Department of Food, Agriculture, and Bioresources, School of Environment, Resources, and Development, Asian Institute of Technology, P.O. Box 4, Klong Luang, Pathum Thani, 12120, Thailand

    Saugat Prajapati, Sushil Koirala & Anil Kumar Anal

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  1. Saugat Prajapati
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  2. Sushil Koirala
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  3. Anil Kumar Anal
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Contributions

All authors contributed to the study conception and design. Conceptualization: Saugat Prajapati, Sushil Koirala, Anil Kumar Anal. Methodology: Saugat Prajapati, Anil Kumar Anal. Formal analysis and investigation: Saugat Prajapati, Anil Kumar Anal. Writing—original draft preparation: Saugat Prajapati, Sushil Koirala. Writing—review and editing: Sushil Koirala, Anil Kumar Anal. Supervision: Anil Kumar Anal. All authors read and approved the final manuscript.

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Correspondence to Anil Kumar Anal.

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Prajapati, S., Koirala, S. & Anal, A.K. Bioutilization of Chicken Feather Waste by Newly Isolated Keratinolytic Bacteria and Conversion into Protein Hydrolysates with Improved Functionalities. Appl Biochem Biotechnol 193, 2497–2515 (2021). https://doi.org/10.1007/s12010-021-03554-4

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