Abstract
The design and fabrication of temperature measurement systems that facilitate successful realization of DNA amplification using a lab-on-a-disc (LOD) device are a highly challenging task. The major challenge lies in the fact that such a system must be directly attached to a heating chamber in a way that enables the accurate measurement of temperature of the chamber while allowing the LOD to rotate. This paper presents a temperature control system for implementing isothermal amplification of DNA samples using an LOD device. The proposed system utilizes a thin-film phase change material and non-contact heating system to remotely measure the actual temperature of the chamber and, if required, rapidly heat it to the desired temperature. The results of the experiments performed in this study demonstrate that the proposed system provides an automated platform for molecular amplification and exhibits an operational performance comparable to that of traditional microcentrifuge tube-based isothermal amplification systems.
Similar content being viewed by others
References
Madou, M., Zoval, J., Jia, G., Kido, H., Kim, J., & Kim, N. (2006). Lab on a CD. Annual Review of Biomedical Engineering, 8(1), 601–628.
Gopinath, S. C., Awazu, K., Tominaga, J., & Kumar, P. K. (2008). Monitoring biomolecular interactions on a digital versatile disk: a BioDVD platform technology. ACS Nano, 2(9), 1885–1895.
Mark, D., Weber, P., Lutz, S., Focke, M., Zengerle, R., & von, S. F. (2011). Aliquoting on the centrifugal microfluidic platform based on centrifugo-pneumatic valves. Microfluidics and Nanofluidics, 10(6), 1279–1288.
Glass, N. R., Shilton, R. J., Chan, P. P., Friend, J. R., & Yeo, L. Y. (2012). Miniaturized lab-on-a-disc (miniLOAD). Small, 8(12), 1881–1888.
Agrawal, S., Morarka, A., Bodas, D., & Paknikar, K. M. (2012). Multiplexed detection of waterborne pathogens in circular microfluidics. Applied Biochemistry and Biotechnology, 167(6), 1668–1677.
Park, J., Sunkara, V., Kim, T. H., Hwang, H., & Cho, Y. K. (2012). Lab-on-a-disc for fully integrated multiplex immunoassays. Analytical Chemistry, 84(5), 2133–2140.
Gorkin, R., Park, J., Siegrist, J., Amasia, M., Lee, B. S., Park, J. M., & Cho, Y. K. (2010). Centrifugal microfluidics for biomedical applications. Lab on a Chip, 10(14), 1758–1773.
Grumann, M., Geipel, A., Riegger, L., Zengerle, R., & Ducrée, J. (2005). Batch-mode mixing on centrifugal microfluidic platforms. Lab on a Chip, 5(5), 560–565.
Ducrée, J., Haeberle, S., Brenner, T., Glatzel, T., & Zengerle, R. (2006). Patterning of flow and mixing in rotating radial microchannels. Microfluidics and Nanofluidics, 2(2), 97–105.
Roy, E., Stewart, G., Mounier, M., Malic, L., Peytavi, R., Clime, L., & Veres, T. (2015). From cellular lysis to microarray detection, an integrated thermoplastic elastomer (TPE) point of care lab on a disc. Lab on a Chip, 15(2), 406–416.
Park, J. M., Cho, Y. K., Lee, B. S., Lee, J. G., & Ko, C. (2007). Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices. Lab on a Chip, 7(5), 557–564.
Steigert, J., Grumann, M., Brenner, T., Riegger, L., Harter, J., Zengerle, R., & Ducrée, J. (2006). Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform. Lab on a Chip, 6(8), 1040–1044.
Wang, G., Ho, H. P., Chen, Q., Yang, A. K. L., Kwok, H. C., Wu, S. Y., & Zhang, X. (2013). A lab-in-a-droplet bioassay strategy for centrifugal microfluidics with density difference pumping, power to disc and bidirectional flow control. Lab on a Chip, 13(18), 3698–3706.
Tachibana, H., Saito, M., Shibuya, S., Tsuji, K., Miyagawa, N., Yamanaka, K., & Tamiya, E. (2015). On-chip quantitative detection of pathogen genes by autonomous microfluidic PCR platform. Biosensors and Bioelectronics, 74, 725–730.
DuVall, J. A., Le, R. D., Tsuei, A. C., Thompson, B. L., Birch, C., Li, J., & Storts, D. R. (2016). A rotationally-driven polyethylene terephthalate microdevice with integrated reagent mixing for multiplexed PCR amplification of DNA. Analytical Methods, 8(40), 7331–7340.
Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research, 28(12), e63–e63.
Moghimi, H., Moradi, A., Hamedi, J., & Basiri, M. (2016). Development of a loop-mediated isothermal amplification assay for rapid and specific identification of ACT producing Alternaria alternata, the agent of brown spot disease in tangerine. Applied Biochemistry and Biotechnology, 178(6), 1207–1219.
Mori, Y., Nagamine, K., Tomita, N., & Notomi, T. (2001). Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochemical and Biophysical Research Communications, 289(1), 150–154.
Kim, T. H., Park, J., Kim, C. J., & Cho, Y. K. (2014). Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. Analytical Chemistry, 86(8), 3841–3848.
Phaneuf, C., Light, Y. K., Tran, H., Singh, A. K., Koh, C. Y. (2015). Non-contact heating system for a centrifugal microlfuidic platform (no. SAND2015-8608C), Sandia National Laboratories (SNL-CA), Livermore, CA (United States).
Sayad, A. A., Ibrahim, F., Uddin, S. M., Pei, K. X., Mohktar, M. S., Madou, M., & Thong, K. L. (2016). A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection. Sensors and Actuators B Chemical, 227, 600–609.
Loo, J. F. C., Kwok, H. C., Leung, C. C. H., Wu, S. Y., Law, I. L. G., Cheung, Y. K., & Kong, S. K. (2017). Sample-to-answer on molecular diagnosis of bacterial infection using integrated lab-on-a-disc. Biosensors and Bioelectronics, 93, 212–219.
Lim, D., & Yoo, J. C. (2017). Chemical cell lysis system applicable to lab-on-a-disc. Applied Biochemistry and Biotechnology, 183(1), 20–29.
Cho, A. R., Dong, H. J., & Cho, S. (2013). Rapid and sensitive detection of Salmonella spp. by using a loop-mediated isothermal amplification assay in duck carcass sample. Korean Journal for Food Science of Animal Resources, 33(5), 655–663.
Sarı, A. (2004). Form-stable paraffin/high density polyethylene composites as solid–liquid phase change material for thermal energy storage: preparation and thermal properties. Energy Conversion and Management, 45(13–14), 2033–2042.
Yoo, J. C. (2016). Bio drive apparatus, and assay method using the same, Patent No: US 9,279,818 B2 (Mar. 8).
Kuniyuki, K. (2007). Optical disk apparatus and a sliding driving mechanism for an optical pickup thereof, Patent No: US 7,266,060 B2 (Sep. 4).
Damien, K., Mary, O.’. S., & Jens, D. (2014). Optical detection strategies for centrifugal microfluidic platforms. Journal of Modern Optics, 61(2), 85–101.
Chen, Z., Zhang, K., Yin, H., Li, Q., Wang, L., & Liu, Z. (2015). Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method. Food Science and Human Wellness, 4(2), 75–79.
Choi, M. S., & Yoo, J. C. (2015). Automated centrifugal-microfluidic platform for DNA purification using laser burst valve and coriolis effect. Applied Biochemistry and Biotechnology, 175(8), 3778–3787.
Swinehart, D. F. (1962). The beer-lambert law. Journal of Chemical Education, 39(7), 333.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Ko, J., Yoo, JC. Loop-Mediated Isothermal Amplification Using a Lab-on-a-Disc Device with Thin-film Phase Change Material. Appl Biochem Biotechnol 186, 54–65 (2018). https://doi.org/10.1007/s12010-018-2720-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-018-2720-8