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Chemical Cell Lysis System Applicable to Lab-on-a-Disc

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Abstract

The design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (LOD). The proposed system contains a sample inlet, phase change material (PCM) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. Compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the LOD and reduced processing time and labor. The experiments are conducted with Salmonella typhimurium strains to demonstrate the performance. The experimental results show that the proposed approach is greatly effective in realizing a chemical cell lysis system on an LOD with higher throughput in terms of purity and yield of DNA.

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Acknowledgements

All authors acknowledge Mathpower Co., Ltd., Korea, for financial support.

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Authors and Affiliations

  1. College of Information & Communication Engineering, SungKyunKwan University, Suwon, South Korea

    Dayeseul Lim & Jae Chern Yoo

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  1. Dayeseul Lim
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  2. Jae Chern Yoo
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Correspondence to Jae Chern Yoo.

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Lim, D., Yoo, J.C. Chemical Cell Lysis System Applicable to Lab-on-a-Disc. Appl Biochem Biotechnol 183, 20–29 (2017). https://doi.org/10.1007/s12010-017-2428-1

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  • DOI: https://doi.org/10.1007/s12010-017-2428-1

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