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Stryphnodendron adstringens (“Barbatimão”) Leaf Fraction: Chemical Characterization, Antioxidant Activity, and Cytotoxicity Towards Human Breast Cancer Cell Lines

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Abstract

We evaluated the chemical composition, antioxidant activity, and antitumor potential of a fraction that was isolated from Stryphnodendron adstringens (barbatimão) leaf aqueous extract. Fraction is composed by gallic acid, procyanidin dimer B1, and (−)-epicatechin-3-O-gallate and it exhibits antioxidant and cytotoxic activities. Fraction was cytotoxic against two human breast cancer cell lines, ER (+) and MCF-7 and the triple-negative, MDA-MB-435. The sulforhodamine B assay showed that, as compared to normal control cells, the fraction significantly (P < 0.05) decreased cancer cell viability. The morphological alterations noted in the treated cancer cells were cell rounding-up, shrinkage, and nuclear condensation reduction of cell diameter and length. Treatment with fraction increased cancer cell expression of Bax, caspase-9, active caspase-3, caspase-8, LC-3, and beclin-1 and decreased Bcl-2, caspase-3, and pro-caspase-8 expression. Altogether, fraction is cytotoxic to both breast cancer cell lines, induces cell death, and its mechanism of action seems to include the induction of apoptosis. Our data support a positive role of the fraction as a chemopreventive agent for antineoplastic drug development.

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Acknowledgements

Grant received from FAPEMIG (number 1305-06), to undertake this work is duly acknowledged. Fellowships from CAPES (APLS), UNIFAL-MG (LMSE), CNPQ (ACFM and CB), FAPEMIG (TNM), and PET-MEC-SESu (CMCPG) were gratefully acknowledged.

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  1. Instituto de Ciências da Natureza, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700, Alfenas, MG, 37130-000, Brazil

    A. P. L. Sabino, L. M. S. Eustáquio, A. C. F. Miranda, C. Biojone, T. N. Mariosa & Cibele Marli Cação Paiva Gouvêa

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  1. A. P. L. Sabino
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Correspondence to Cibele Marli Cação Paiva Gouvêa.

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Rat primary bone marrow (RPBM) cell culture (normal control cells) was established from femur of 120-day-old Wistar rats, after approval from the UNIFAL-MG Commission for Animal Experimentation (process n. 338).

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Sabino, A.P.L., Eustáquio, L.M.S., Miranda, A.C.F. et al. Stryphnodendron adstringens (“Barbatimão”) Leaf Fraction: Chemical Characterization, Antioxidant Activity, and Cytotoxicity Towards Human Breast Cancer Cell Lines. Appl Biochem Biotechnol 184, 1375–1389 (2018). https://doi.org/10.1007/s12010-017-2632-z

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