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Isolation and anticancer activity of quercetin from Acalypha indica L. against breast cancer cell lines MCF-7 and MDA-MB-231

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Abstract

In this study, the active components of the plant were carefully extracted and identified using three solvent systems. After extraction, we used solvent systems to further purify the main flavonoid chemical constituent. As a result of our analytical strategy, which included HPLC analysis, MS/MS spectroscopic analysis, and NMR data-based constructions, quercetin was determined to be the main chemical constituent. Our study suggests the potential therapeutic advantages of quercetin, a compound found in the leaves of Acalypha indica, for treating breast cancer cell lines MCF-7 and MDA-MB-231. Our comparison of Quercetin to the regularly prescribed medicine Doxorubicin shows that it has the capacity to inhibit MCF-7 and MDA-MB-231 cells. Measurements of apoptosis and cell cycle phase showed this to be the case. Furthermore, a ladder that formed as a result of cellular damage brought on by ROS provided further proof of the drug's impact on DNA integrity. Notably, pro-apoptotic proteins displayed increased apoptosis activity in cells treated with quercetin. Given that it is extracted from plants and has less adverse effects than other compounds, quercetin is a viable option for further clinical study. The objective is to fight breast cancer, one of the most prevalent diseases in the world and a main cause of death for women. Thus, our research makes a significant addition to the ongoing search for potent, plant-based breast cancer treatments.

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Acknowledgements

The authors would like to thank for the support from the Department of Genetics, Osmania University, for providing the resources and moral support to complete this research.

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This research received no specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. Sudhakar Chekuri and Satyanarayana Swamy Vyshnava have contributed equally to this work.

Authors and Affiliations

  1. Department of Genetics and Biotechnology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India

    Sudhakar Chekuri, Sai Bindu Karamthote Cheniya & Roja Rani Anupalli

  2. Department of Biotechnology, University College of Science, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, 515003, India

    Satyanarayana Swamy Vyshnava

  3. Department of Microbiology, Keshav Memorial Institute of Commerce and Sciences, Narayanguda, Hyderabad, Telangana, 500029, India

    Satyanarayana Swamy Vyshnava

  4. Department of Microbiology, Government Medical College and General Hospital, Suryapet, Telangana, 508213, India

    Swarupa Lakshmi Somisetti

  5. Bogomolets National Medical University, Taras Shevchenko Blvd 13, Kiev, 01601, Ukraine

    Chakradhar Gandu

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  1. Sudhakar Chekuri
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Contributions

SC, and SSV equally contributed in this research and involved in Conceptualization, Methodology, Investigation, Formal analysis, Writing—Original Draft, SSV managed to curate the software applications, Validation, Formal analysis, Investigation, Resources was provided by Ms. SLS, and Prof. RRA, Data Curation was done by Ms. SBKC, and Mr. CG and finally Supervision, Project administration, Writing—Review and Editing of this research was supported by Prof. RRA.

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Correspondence to Roja Rani Anupalli.

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Chekuri, S., Vyshnava, S.S., Somisetti, S.L. et al. Isolation and anticancer activity of quercetin from Acalypha indica L. against breast cancer cell lines MCF-7 and MDA-MB-231. 3 Biotech 13, 289 (2023). https://doi.org/10.1007/s13205-023-03705-w

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