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Simultaneous Bioconversion of Xylose and Glycerol to Xylonic Acid and 1,3-Dihydroxyacetone from the Mixture of Pre-Hydrolysates and Ethanol-Fermented Waste Liquid by Gluconobacter oxydans

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Abstract

Simultaneous bioconversion of xylose and glycerol to xylonic acid and 1,3-dihydroxyacetone (DHA) was realized by using Gluconobacter oxydans (G. oxydans). Currently, the enzymatic hydrolysate to ethanol-fermented waste liquid and the inorganic acid pre-hydrolysate that contain abundant glycerol and xylose were difficult to be utilized or disposed. Based on the method of compressed oxygen supply-sealed and stirred tank reactor system (COS-SSTR), the xylonic acid and 1,3-dihydroxyacetone could be co-produced rapidly with the mixture of the dilute sulfuric acid pre-hydrolysate and ethanol-fermented waste liquid of enzymatic hydrolysate (MPEW) as material. By means of the system, we finally produced 102.3 ± 3.2 g/L xylonic acid and 40.6 ± 1.8 g/L 1,3-dihydroxyacetone at yield of 92.4 ± 2.8 % and 80.6 ± 3.5 % directly and simultaneously from the mixed solution. The central features of this bioprocess application would enable cost-competitive bacterial xylonic acid and 1,3-dihydroxyacetone production from lignocellulosic materials.

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Acknowledgments

The research was supported by the National Natural Science Funding of China (31370573) and Key Research and Development Program of Jiangsu (BE2015758). Also, the authors gratefully acknowledge financial support from Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Xin Zhou, Yong Xu & Shiyuan Yu

  2. Jiangsu Province Key Laboratory of Green Biomass Based Fuel Chemical of, Nanjing, 210037, People’s Republic of China

    Xin Zhou, Yong Xu & Shiyuan Yu

Authors
  1. Xin Zhou
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  2. Yong Xu
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  3. Shiyuan Yu
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Correspondence to Yong Xu.

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Zhou, X., Xu, Y. & Yu, S. Simultaneous Bioconversion of Xylose and Glycerol to Xylonic Acid and 1,3-Dihydroxyacetone from the Mixture of Pre-Hydrolysates and Ethanol-Fermented Waste Liquid by Gluconobacter oxydans . Appl Biochem Biotechnol 178, 1–8 (2016). https://doi.org/10.1007/s12010-015-1853-2

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  • DOI: https://doi.org/10.1007/s12010-015-1853-2

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