Skip to main content
SpringerLink
Log in

Isolation, Purification, and Anti-Aging Activity of Melanin from Lachnum singerianum

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Intracellular melanin from Lachnum singerianum YM296 (LIM) was obtained by the method of microwave-assisted extraction. On the basis of single factor experiment, the optimum microwave-assisted extraction conditions of LIM using Box–Behnken design were as follows: NaOH concentration, 1.05 mol/L; ratio of raw material to liquid ratio, 1:14.72 (g/mL); microwave time, 118.70 s; and microwave power, 320 W. Under these conditions, the extraction yield of LIM was 11.08 %, and 40.43 % higher than that of extraction by alkali extraction and acid precipitation. The results showed that microwave-assisted extraction could increase the extraction yield of LIM. Three homogeneous components were fractionated from LIM by Sephadex G-15 column. LIM-a was the main homogeneous component, accounting for 75.7 % of the total content of the homogeneous components. Aged mice model were induced by injecting d-galactose, and the aged mice were given LIM-a of different dosages, respectively. The results showed that LIM-a can significantly increase body weight, thymus indexes, and spleen indexes of the aged mice; effectively elevate the levels of SOD, GSH-PX, and CAT; and decrease the level of MDA in mice liver homogenate, brain homogenate, and serum, indicating that LIM-a had significant anti-aging activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Argyropoulou, A., Aligiannis, N., & Trougakos, I. P. (2013). Natural compounds with anti-ageing activity. Natural Product Reports, 30, 1412–1437.

    Article  CAS  Google Scholar 

  2. Harman, D. (2006). Free radical theory of aging: an update. Annals of the New York Academy of Sciences, 1067, 10–21.

    Article  CAS  Google Scholar 

  3. Bonnefoy, M., Dmi, J., & KoStka, T. (2002). Antioxidants to slow aging, facts and perpectives. Press Medical, 31, 1174–1184.

    Google Scholar 

  4. Aghajanyan, A. E., Hambardzumyan, A. A., Hovsepyan, A. S., Asaturian, R. A., Vardanyan, A. A., & Saghiyan, A. A. (2005). Isolation, purification and physicochemical characterization of water-soluble Bacillus thuringiensis melanin. Pigment Cell Research, 18, 130–135.

    Article  CAS  Google Scholar 

  5. Gómez-Marín, A. M., & Sánchez, C. I. (2010). Thermal and mass spectroscopic characterization of a sulphur-containing bacterial melanin from Bacillus subtilis. Journal of Non-Crystalline Solids, 356, 1576–1580.

    Article  Google Scholar 

  6. Tu, Y. G., Sun, Y. Z., Tian, Y. G., Xie, M. Y., & Chen, J. (2009). Physicochemical characterisation and antioxidant activity of melanin from the muscles of Taihe black-bone silky fowl (Gallus gallus domesticus Brisson). Food Chemistry, 114, 1345–1350.

    Article  CAS  Google Scholar 

  7. Wang, H. S., Pan, Y. M., Tang, X. J., & Huang, Z. Q. (2006). Isolation and characterization of melanin from Osmanthus fragrans’ seeds. LWT-Food Science and Technology, 39, 496–502.

    Article  CAS  Google Scholar 

  8. Dong, C., & Yao, Y. (2012). Isolation, characterization of melanin derived from Ophiocordyceps sinensis, an entomogenous fungus endemic to the Tibetan Plateau. Journal of Bioscience and Bioengineering, 113, 474–479.

    Article  CAS  Google Scholar 

  9. El-Obeid, A., Al-Harbi, S., Al-Jomah, N., & Hassib, A. (2006). Herbal melanin modulates tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) production. Phytomedicine, 13, 324–333.

    Article  CAS  Google Scholar 

  10. Manninga, J. T., Bundredb, P. E., & Henzi, P. (2006). Melanin and HIV in sub-Saharan Africa. Journal of Theoretical Biology, 223, 131–133.

    Article  Google Scholar 

  11. Sava, V. M., Hung, Y. C., Blagodarsky, V. A., Hong, M. Y., & Huang, G. S. (2003). The liver-protecting activity of melanin-like pigment derived from black tea. Food Research International, 36, 505–511.

    Article  CAS  Google Scholar 

  12. Li, J., Zhang, Q. H., Lan, J. J., & Wang, J. Z. (2012). Research of extraction technology of the melanin from core-shell of wild apricots using response surface analysis. Science and Technology of Food Industry, 33, 295–299.

    Google Scholar 

  13. Liu, Y., & Simon, J. D. (2003). The effect of preparation procedures on the morphology of melanin from the ink sac of Sepia officinalis. Pigment Cell Research, 16, 72–80.

    Article  Google Scholar 

  14. Dong, H. Q., Huang, J. B., Dong, H. Q., & Zhang, Y. H. (2012). Ultrasonic-assisted extraction of melanin from flowers of Mucuna birdwoodiana tutch. Food Research and Development, 33, 140–143.

    CAS  Google Scholar 

  15. Hu, Z. J., & Cai, Z. H. (2007). Study on extraction technology of black pigment from Vaccinium bracteatum thunb. leaf under microwave radiation. Chinese Wild Plant Resources, 26, 52.

    Google Scholar 

  16. Song, R., Zhu, Y., & Yu, Q. D. (2009). Identification and physico-chemical properties of squid melanin from squid ink by enzymatic hydrolysis. Journal of Zhejiang Ocean University (Natural Science), 28, 95–98.

    CAS  Google Scholar 

  17. Ye, M., Guo, G. Y., Lu, Y., Song, S., Wang, H. Y., & Yang, L. (2014). Purification, structure and anti-radiation activity of melanin from Lachnum YM404. International Journal of Biological Macromolecules, 63, 170–176.

    Article  CAS  Google Scholar 

  18. Ye, M., Wang, Y., Guo, G. Y., He, Y. L., Lu, Y., & Ye, Y. W. (2012). Physicochemical characteristics and antioxidant activity of arginine-modified melanin from Lachnum YM-346. Food Chemistry, 135, 2490–2497.

    Article  CAS  Google Scholar 

  19. Zhuang, W. Y. (2004). Flora fungorum sinicorum. Hyaloscyphaceae, Sarcoscyphaceae et Sarcosomataceae. Science, 21, 75–76.

    Google Scholar 

  20. Choi, Y. W., Hyde, K. D., & Ho, W. H. (1999). Single spore isolation of fungi. Fungal Diversity, 3, 29–38.

    Google Scholar 

  21. Ye, M., Chen, X., Li, G. W., Guo, G. Y., & Yang, L. (2011). Structural characteristics of pheomelanin-like pigment from Lachnum singerianum. Advanced Materials Research, 284, 1742–1745.

    Article  Google Scholar 

  22. Olennikov, D. N., Agafonova, S. V., Stolbikova, A. V., & Rokhin, A. V. (2011). Melanin of Laetiporus sulphureus (Bull.: Fr) murr sterile form. Applied Biochemistry and Microbiology, 47, 29–30.

    Google Scholar 

  23. Ye, M., Chen, W. X., Qiu, T., Yuan, R. Y., Ye, Y. W., & Cai, J. M. (2012). Structural characterisation and anti-ageing activity of extracellular polysaccharide from a strain of Lachnum sp. Food Chemistry, 132, 338–343.

    Article  CAS  Google Scholar 

  24. Zhang, Q. A., Zhang, Z. Q., Yue, X. F., Fan, X. H., Li, T., & Chen, S. F. (2009). Response surface optimization of ultrasound-assisted oil extraction from autoclaved almond powder. Food Chemistry, 116, 513–518.

    Article  CAS  Google Scholar 

  25. Wang, Y., Song, S., Ye, M., Lu, Y., Li, L., & Li, J. H. (2013). Extraction technology, structural characteristics and antibacterial activity of melanin from a strain of Lachnum. Journal of Pure and Applied Microbiology, 7, 285–292.

    CAS  Google Scholar 

  26. Uddin, M. N., Nishio, N., Ito, S., Suzuki, H., & Isobe, K. (2010). Toxic effects of d-galactose on thymus and spleen that resemble aging. Journal of Immunotoxicology, 7, 165–173.

    Article  CAS  Google Scholar 

  27. Meng, J. X., Yang, P., & Zhang, L. (2010). Effect of eleutheroside on the immune function in aging mice caused by d-galactose. Chinese Journal of Gerontology, 30, 216–218.

    CAS  Google Scholar 

  28. Zhen, T. Y., Zhao, M. X., & Lei, M. (2012). Effect of squid melanin on antioxidant function of subacute aging mice. Journal of Chinese Institute of Food Science and Technology, 12, 61–65.

    CAS  Google Scholar 

  29. Lin, X., Huang, Z., Chen, X., Rong, Y., Zhang, S., & Jiao, Y. (2014). Protective effect of Millettia pulchra polysaccharide on cognitive impairment induced by d-galactose in mice. Carbohydrate Polymers, 101, 533–543.

    Article  CAS  Google Scholar 

  30. Zhang, Z., Lv, G., Pan, H., Pandey, A., He, W., & Fan, L. (2012). Antioxidant and hepatoprotective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey. International Journal of Biological Macromolecules, 51, 1140–1146.

    Article  CAS  Google Scholar 

  31. Xing, H., Li, S., Wang, Z., Gao, X., Xu, S., & Wang, X. (2012). Histopathological changes and antioxidant response in brain and kidney of common carp exposed to atrazine and chlorpyrifos. Chemosphere, 88, 377–383.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31270060), Annual Research Projects of Anhui Province (12070303037), and the SME Technology Innovation Foundation (12C26213403307).

Author information

Authors and Affiliations

  1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China

    Ying Lu, Ming Ye, Sheng Song, Lan Li, Farnaz Shaikh & Junhong Li

Authors
  1. Ying Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ming Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sheng Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Farnaz Shaikh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Junhong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ming Ye.

Additional information

Highlights

The optimum microwave-assisted extraction conditions of Lachnum singerianum were obtained. The yield of melanin (LIM) under the optimal conditions was 11.08 %. Three homogeneous components were fractionated from L. singerianum intracellular melanins. LIM-a was the main homogeneous component. LIM-a had significant anti-aging activity.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lu, Y., Ye, M., Song, S. et al. Isolation, Purification, and Anti-Aging Activity of Melanin from Lachnum singerianum . Appl Biochem Biotechnol 174, 762–771 (2014). https://doi.org/10.1007/s12010-014-1110-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-1110-0

Keywords

Search

Navigation