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Lipid Production by Cryptococcus curvatus on Hydrolysates Derived from Corn Fiber and Sweet Sorghum Bagasse Following Dilute Acid Pretreatment

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Abstract

Corn fiber and sweet sorghum bagasse (SSB) are both pre-processed lignocellulosic materials that can be used to produce liquid biofuels. Pretreatment using dilute sulfuric acid at a severity factor of 1.06 and 1.02 released 83.2 and 86.5 % of theoretically available sugars out of corn fiber and SSB, respectively. The resulting hydrolysates derived from pretreatment of SSB at SF of 1.02 supported growth of Cryptococcus curvatus well. In 6 days, the dry cell density reached 10.8 g/l with a lipid content of 40 % (w/w). Hydrolysates from corn fiber, however, did not lead to any significant cell growth even with addition of nutrients. In addition to consuming glucose, xylose, and arabinose, C. curvatus also utilized formic acid, acetic acid, 4-hydroxymethylfurfural, and levulinic acid for growth. Thus, C. curvatus appeared to be an excellent yeast strain for producing lipids from hydrolysates developed from lignocellulosic feedstocks.

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Acknowledgments

Kimberly Jarosz thankfully acknowledges the opportunity and financial support from the McNair Scholars Program at SIUC. Ashley T. Wardlow from Florida International University appreciates support from a NSF REU program (DMR 1157058) at SIUC. We also thank Dr. Sabrina Trupia at National Corn-to-ethanol Research Center for providing the corn fiber samples.

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Authors and Affiliations

  1. Department of Civil & Environmental Engineering, Southern Illinois University Carbondale, 1230 Lincoln Dr., Carbondale, IL, 62901, USA

    Yanna Liang, Kimberly Jarosz, Ji Zhang & Yi Cui

  2. Florida International University, 11200 SW 8th St., OE 304, Miami, FL, 33199, USA

    Ashley T. Wardlow

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  1. Yanna Liang
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  2. Kimberly Jarosz
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  3. Ashley T. Wardlow
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  4. Ji Zhang
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  5. Yi Cui
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Correspondence to Yanna Liang.

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Liang, Y., Jarosz, K., Wardlow, A.T. et al. Lipid Production by Cryptococcus curvatus on Hydrolysates Derived from Corn Fiber and Sweet Sorghum Bagasse Following Dilute Acid Pretreatment. Appl Biochem Biotechnol 173, 2086–2098 (2014). https://doi.org/10.1007/s12010-014-1007-y

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  • DOI: https://doi.org/10.1007/s12010-014-1007-y

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