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Kinetics of Enzymatic Hydrolysis of Olive Oil in Batch and Fed-batch Systems

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Abstract

This work reports experimental data, kinetic modeling, and simulations of enzyme-catalyzed hydrolysis of olive oil. This reaction was performed in batch system and an ordered-sequential Bi Bi model was used to model the kinetic mechanism. A fed-batch system was proposed and experimental data were obtained and compared to the simulated values. The kinetic model used was able to correlate the experimental data, in which a satisfactory agreement between the experimental data and modeling results was obtained under different enzyme concentration and initial free water content. Therefore, the modeling allowed a better understanding of the reaction kinetics and affords a fed-batch simulation for this system. From the results obtained, it was observed that the fed-batch approach showed to be more advantageous when compared to the conventional batch system.

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Abbreviations

DAG:

Diacylglycerol

FFA:

Free fatty acid

G:

Glycerol

H2O:

Water in the oil phase

H2Oins :

Water not solubilized in the oil

K r (r = 1,…,7):

Apparent rate constants (g-substrate2/mmol2)

MAG:

Monoacylglycerol

NOBS:

Number of observations

M FFA :

Molar mass of the free fatty acid of olive oil (g/mol)

M DAG :

Molar mass of the diacylglycerol of olive oil (g/mol)

M MAG :

Molar mass of the monoacylglycerol of olive oil (g/mol)

M TAG :

Molar mass of the triacylglycerol of olive oil (g/mol)

rmsd:

Root-mean-square deviation

TAG:

Triacylglycerol

U r (r = 1,…,6):

Apparent rate constants (g-substrate/(h mmol))

[x oilsurf ]:

Molar fraction of surfactants in the oil

[w expio ]:

Experimental mass fraction of pseudo-component i in the observation o

[w calcio ]:

Calculated mass fraction of pseudo-component i in the observation o

[DAG]:

Diacylglycerol concentration (mmol/g-substrate)

[FFA]:

Free fatty acids concentration (mmol/g-substrate)

[H2O]:

Water concentration in the oil phase (mmol/g-substrate)

[H2O]0 :

Initial water concentration in the oil phase (mmol/g-substrate)

[H2Oins]:

Concentration of water not solubilized in the oil phase (mmol/g-substrate)

[G]:

Glycerol concentration (mmol/g-substrate)

[MAG]:

Monoacylglycerol concentration (mmol/g-substrate)

[TAG]:

Triacylglycerol concentration (mmol/g-substrate)

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Acknowledgments

The authors thank the CNPq, PRH24/ANP, CAPES, and Fundação Araucária (Brazilian Agencies) for financial support and scholarships.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Paraná (UFPR), Curitiba, 82530-990, PR, Brazil

    Paloma Souza Cabral, Arion Zandoná Filho, Fernando Augusto Pedersen Voll & Marcos Lúcio Corazza

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  1. Paloma Souza Cabral
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  2. Arion Zandoná Filho
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  3. Fernando Augusto Pedersen Voll
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  4. Marcos Lúcio Corazza
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Correspondence to Marcos Lúcio Corazza.

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Cabral, P.S., Filho, A.Z., Voll, F.A.P. et al. Kinetics of Enzymatic Hydrolysis of Olive Oil in Batch and Fed-batch Systems. Appl Biochem Biotechnol 173, 1336–1348 (2014). https://doi.org/10.1007/s12010-014-0943-x

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