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Effect of Cycle Time on Polyhydroxybutyrate (PHB) Production in Aerobic Mixed Cultures

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Abstract

The aim of this study was to investigate the effect of cycle time on polyhydroxybutyrate (PHB) production under aerobic dynamic feeding system. The acetate-fed feast and famine sequencing batch reactor was used to enrich PHB accumulating microorganism. Sequencing batch reactor (SBR) was operated in four different cycle times (12, 8, 4, and 2 h) fed with a synthetic wastewater. The system performance was determined by monitoring total dissolved organic carbon, dissolved oxygen, oxidation-reduction potential, and PHB concentration. In this study, under steady-state conditions, the feast period of the SBR was found to allow the PHB storage while a certain part of stored PHB was used for continued growth in famine period. The percentage PHB storages by aerobic microorganism were at 16, 18, 42, and 55 % for the 12, 8, 4, and 2-h cycle times, respectively. The PHB storage was increased as the length of the cycle time was decreased, and the ratio of the feast compared to the total cycle length was increased from around 13 to 33 % for the12 and 2-h cycle times, respectively.

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Authors and Affiliations

  1. Department of Bioengineering and Science, Kahramanmaras Sutcu Imam University, Kahramanmaras, 46100, Turkey

    Sebnem Ozdemir & Dilek Akman

  2. Department of Environmental Engineering, Kahramanmaras Sutcu Imam University, Kahramanmaras, 46100, Turkey

    Kevser Cirik & Ozer Cinar

  3. Bioengineering and Genetics Program, International University of Sarajevo, Hrasnicka Cesta 15, 71210, Sarajevo, Bosnia and Herzegovina

    Ozer Cinar

Authors
  1. Sebnem Ozdemir
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  2. Dilek Akman
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  3. Kevser Cirik
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  4. Ozer Cinar
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Correspondence to Ozer Cinar.

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Ozdemir, S., Akman, D., Cirik, K. et al. Effect of Cycle Time on Polyhydroxybutyrate (PHB) Production in Aerobic Mixed Cultures. Appl Biochem Biotechnol 172, 2390–2399 (2014). https://doi.org/10.1007/s12010-013-0676-2

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  • DOI: https://doi.org/10.1007/s12010-013-0676-2

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