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Bioproduction of Food Additives Hexanal and Hexanoic Acid in a Microreactor

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Abstract

Hexanal and hexanoic acid have number of applications in food and cosmetic industry because of their organoleptic characteristics. Problems like low yields, formation of unwanted by-products, and large quantities of waste in their traditional production processes are the reasons for developing new production methods. Biotransformation in a microreactor, as an alternative to classical synthesis processes, is being investigated. Because conditions in microreactors can be precisely controlled, the quality of the product and its purity can also be improved. Biocatalytic oxidation of hexanol to hexanal and hexanoic acid using suspended and immobilized permeabilized whole baker’s yeast cells and suspended and immobilized purified alcohol dehydrogenase (ADH) was investigated in this study. Three different methods for covalent immobilization of biocatalyst were analyzed, and the best method for biocatalyst attachment on microchannel wall was used in the production of hexanal and hexanoic acid.

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Abbreviations

c :

concentration (mmol/dm3)

k d :

enzyme operational stability decay rate constant (1/h)

T :

temperature (°C)

t :

time (h)

V :

reactor volume (mm3)

V.A. :

volumetric activity (U/cm3)

X :

conversion (%)

γ:

mass concentration (g/cm3)

λ:

wavelength (nm)

τ:

residence time (s)

Φ:

flow rate (mm3/min)

ADH :

alcohol dehydrogenase

APTES :

3-aminopropyltrietoxysilane

CTAB :

hexadecyltrimethylammonium bromide

i :

inlet

NAD + :

nicotinamide adenine dinucleotide

NADH :

nicotinamide adenine dinucleotide hydrate

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Acknowledgments

This research was financially supported through the Croatian Science Foundation.

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Authors and Affiliations

  1. Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000, Zagreb, Croatia

    Anita Šalić, Katarina Pindrić & Bruno Zelić

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  1. Anita Šalić
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  2. Katarina Pindrić
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  3. Bruno Zelić
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Correspondence to Bruno Zelić.

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Šalić, A., Pindrić, K. & Zelić, B. Bioproduction of Food Additives Hexanal and Hexanoic Acid in a Microreactor. Appl Biochem Biotechnol 171, 2273–2284 (2013). https://doi.org/10.1007/s12010-013-0495-5

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  • DOI: https://doi.org/10.1007/s12010-013-0495-5

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