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Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli

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Abstract

Steam-exploded corn stalk biomass was used as the substrate for succinic acid production via lignocellulose enzymatic hydrolysis and fermentation. Succinic acid fermentation was investigated in Escherichia coli strains overexpressing cyanobacterium Anabaena sp. 7120 ecaA gene encoding carbonic anhydrase (CA). For the washed steam-exploded corn stalk at 30 % substrate concentration, i.e., 30 % water-insoluble solids (WIS), enzymatic hydrolysis yielded 97.5 g/l glucose solution and a cellulose conversion of 73.6 %, thus a high succinic acid level up to 38.6 g/l. With the unwashed steam-exploded corn stalk, though a cellulose conversion of 71.2 % was obtained in hydrolysis at 30 % solid concentration (27.9 % WIS), its hydrolysate did not ferment at all, and the hydrolysate of 25 % solid loading containing 3.8 g/l acetic acid and 168.2 mg/l furfural exerted a strong inhibition on succinic acid production.

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Acknowledgments

Financial support by the National Science Foundation of China (No. 21206175, and 21106191) were gratefully acknowledged.

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Authors and Affiliations

  1. Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong, 510080, People’s Republic of China

    Dexi Wu & Yugang Dong

  2. National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China

    Qiang Li

  3. College of Chemistry and Chemical engineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Dan Wang

Authors
  1. Dexi Wu
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  2. Qiang Li
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  3. Dan Wang
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  4. Yugang Dong
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Corresponding author

Correspondence to Yugang Dong.

Additional information

Dexi Wu and Qiang Li contributed equally to this work.

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Wu, D., Li, Q., Wang, D. et al. Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli . Appl Biochem Biotechnol 170, 1942–1949 (2013). https://doi.org/10.1007/s12010-013-0319-7

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  • DOI: https://doi.org/10.1007/s12010-013-0319-7

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