Abstract
Hexanoic acid production by a bacterium using sucrose as an economic carbon source was studied under conditions in which hexanoic acid was continuously extracted by liquid–liquid extraction. Megasphaera elsdenii NCIMB 702410, selected from five M. elsdenii strains, produced 4.69 g l−1 hexanoic acid in a basal medium containing sucrose. Production increased to 8.19 g l−1 when the medium was supplemented by 5 g l−1 sodium butyrate. A biphasic liquid–liquid extraction system with 10 % (v/v) alamine 336 in oleyl alcohol as a solvent was evaluated in a continuous stirred-tank reactor held at pH 6. Over 90 % (w/w) of the hexanoic acid in a 0.5 M aqueous solution was transferred to the extraction solvent within 10 h. Cell growth was not significantly inhibited by direct contact of the fermentation broth with the extraction solvent. The system produced 28.42 g l−1 of hexanoic acid from 54.85 g l−1 of sucrose during 144 h of culture, and 26.52 and 1.90 g l−1 of hexanoic acid was accumulated in the extraction solvent and the aqueous fermentation broth, respectively. The productivity and yield of hexanoic acid were 0.20 g l−1 h−1 and 0.50 g g−1 sucrose, respectively.
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References
Levy, P. F., Sanderson, J. E., Ashare, E. & de Riel, S. R. (1983). In Wise, D. L. (Ed.), CRC liquid fuels developments (pp. 159–188). Boca Raton: CRC
Aly, M., & Baumgarten, E. (2001). Applied catalysis A: general, 210, 1–12.
Wasewar, K. L., & Shende, D. Z. (2010). Journal of Chemical & Engineering Data, 55, 4121–4125.
Gervajio, G. C. (2005). In Shahidi, F. (Ed.), Bailey’s industrial oil and fat products, vol. 6. New York: Wiley
Kenealy, W. R., Cao, Y., & Weimer, P. J. (1995). Applied Microbiology and Biotechnology, 44, 507–513.
Holdeman, L. V., Cato, E. P., & Moore, W. E. C. (1977). Anaerobe laboratory manual, (4th ed.). Blacksburg: Virginia Polytechnic Institute and State University.
Barker, H. A., & Taha, S. M. (1942). Journal of Bacteriology, 43, 347–363.
Kenealy, W. R., & Waselefsky, D. M. (1985). Archives of Microbiology, 141, 187–194.
Jeon, B. S., Kim, B. C., Um, Y. & Sang, B. I. (2010). Applied Microbiology and Biotechnology, 88, 1161–1167.
Rogosa, M. (1971). International Journal of Systematic Bacteriology, 21, 187–189.
Marounek, M., Fliegrova, K., & Bartos, S. (1989). Applied and Environmental Microbiology, 55, 1570–1573.
Giesecke, D., Wiesmayr, S., & Ledinek, M. (1970). Journal of General Microbiology, 64, 123–126.
Stanton, T. B., & Humphrey, S. B. (2003). Applied and Environmental Microbiology, 69, 3874–3882.
Sugihara, P. T., Sutter, V. L., Attebery, H. R., Bricknell, K. S., & Finegold, S. M. (1974). Applied Microbiology, 27, 274–275.
Soto-Cruz, O., Chavez-Rivera, R., & Saucedo-Castaneda, G. (2001). Brazilian Archives of Biology and Technology, 44, 179–184.
Counotte, G. H., Prins, R. A., Janssen, R. H., & Debie, M. J. (1981). Applied and Environmental Microbiology, 42, 649–655.
Tsukahara, T., Hashizume, K., Koyama, H., & Ushida, K. (2006). Animal Science Journal, 77, 454–461.
Kim, Y. J., Liu, R. H., Rychlik, J. L., & Russell, J. B. (2002). Journal of Applied Microbiology, 92, 976–982.
Khan, M. A. (2006). PhD thesis, Electronic Publication, Victoria University, Melbourne, Australia
Dekishima, Y., Lan, E. I., Shen, C. R., Cho, K. M., & Liao, J. C. (2011). Journal of the American Chemical Society, 133, 11399–11401.
Machado, H. B., Dekishima, Y., Luo, H., Lan, E. I., & Liao, J. C. (2012). Metabolic Engineering, 14, 504–511.
Hino, T., Miyazaki, K., & Kuroda, S. (1991). Journal of General and Applied Microbiology, 37, 121–129.
Russell, J. B. (1992). Journal of Applied Bacteriology, 73, 363–370.
Wasewar, K. L., & Shende, D. Z. (2011). Journal of Chemical & Engineering Data, 56, 288–297.
Roddick, F. A., & Britz, M. L. (1997). Journal of Chemical Technology and Biotechnology, 69, 383–391.
Helsel, R. W. (1977). Chemical Engineering Progress, 73, 55–59.
Kertes, A. S., & King, C. J. (1986). Biotechnology and Bioengineering, 28, 269–282.
Wu, Z., & Yang, S. T. (2003). Biotechnology and Bioengineering, 82, 93–102.
Zigova, J., Sturdik, E., Vandak, D., & Schlosser, S. (1999). Process Biochemistry, 34, 835–843.
Weimer, P. J., & Stevenson, D. M. (2012). Applied Microbiology and Biotechnology, 94, 461–466.
Jeon, B. S., Um, Y. S., Lee, S. M., Lee, S. Y., Kim, H. J., Kim, Y. H., et al. (2008). Energy & Fuels, 22, 83–86.
Paredes, C. J., Alsaker, K. V., & Papoutsakis, E. T. (2005). Nature Reviews Microbiology, 3, 969–978.
Yang, S. T., White, S. A., & Hsu, S. T. (1991). Industrial and Engineering Chemistry Research, 30, 1335–1342.
Romero, C. M., & Suárez, F. (2009). Journal of Solution Chemistry, 38, 315–320.
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This work was supported by the research fund of Hanyang University (HY-2011-N) and by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Education, Science and Technology of Korea (2012053893).
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Kieun Choi and Byoung Seung Jeon contributed equally as first authors to this work.
Youngsoon Um and Byoung-In Sang contributed equally as corresponding authors to this work.
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Choi, K., Jeon, B.S., Kim, BC. et al. In Situ Biphasic Extractive Fermentation for Hexanoic Acid Production from Sucrose by Megasphaera elsdenii NCIMB 702410. Appl Biochem Biotechnol 171, 1094–1107 (2013). https://doi.org/10.1007/s12010-013-0310-3
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DOI: https://doi.org/10.1007/s12010-013-0310-3