Skip to main content
SpringerLink
Log in

Surface Display of Bacterial Metallothioneins and a Chitin Binding Domain on Escherichia coli Increase Cadmium Adsorption and Cell Immobilization

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

To increase the level of adsorption of cadmium ions to the surface of Escherichia coli, we fused cyanobacterial metallothioneins, SmtA (from Synechococcus elongatus PCC 3601) and MtnA (from Synechococcus vulcanus) to the E. coli cell surface using a Lpp′-OmpA-based display system. E. coli strains expressing Lpp′-OmpA–SmtA-linker-ChBD (chitin-binding domain from Bacillus pumillus SG2 chitinase S; chiS) and Lpp′-OmpA–MtnA-linker-ChBD on their surface adsorbed more cadmium compared to the E. coli cells expressing only the Lpp′-OmpA-linker-ChBD hybrid. These constructs also were bound to chitin through their chitin-binding domain, allowing them to be immobilized on a chitin matrix. We assessed surface presentation of Lpp′-OmpA–SmtA-linker-ChBD, Lpp′-OmpA–MtnA-linker-ChBD, and Lpp′-OmpA-linker-ChBD using immunostaining. The Lpp′-OmpA–SmtA-linker-ChBD chimera adsorbed metal and was bound to chitin with the highest efficiency compared to the other chimeras, suggesting that it is an effective bioadsorbent. This is the first example of coupling metal adsorption with cell immobilization using a whole-cell bioadsorbent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Ahmadian, G., Degrassi, G., Venturi, V., Zeigler, D. R., Soudi, M., & Zanguinejad, P. (2007). Journal of Applied Microbiology, 103, 1081–1089.

    Article  CAS  Google Scholar 

  2. Amet, N., Lee, H. F., & Shen, W. C. (2009). Pharmaceutical Research, 26, 523–528.

    Article  CAS  Google Scholar 

  3. Arai, R., Ueda, H., Kitayama, A., Kamiya, N., & Nagamune, T. (2001). Protein Engineering, 14, 529–532.

    Article  CAS  Google Scholar 

  4. Bae, W., Mehra, R. K., Mulchandani, A., & Chen, W. (2001). Applied and Environmental Microbiology, 67, 5335–5338.

    Article  CAS  Google Scholar 

  5. Blindauer, C. A. (2011). The Journal of Biological Inorganic Chemistry, 16, 1011–1024. doi:10.1007/s00775-011-0790-y.

    Article  CAS  Google Scholar 

  6. Daugherty, P. S., Olsen, M. J., Iverson, B. L., & Georgiou, G. (1999). Protein Engineering, 12, 613–621.

    Article  CAS  Google Scholar 

  7. Ecker, D. J., Butt, T. R., Sternberg, E. J., Neeper, M. P., Debouck, T. C., Gormann, J. A., et al. (1986). The Journal of Biological Chemistry, 261, 16895–16900.

    CAS  Google Scholar 

  8. Gadd, G. M., & White, C. (1993). Trends Biotechnology, 11, 353–359.

    Article  CAS  Google Scholar 

  9. George, R. A., & Heringa, J. (2003). Protein Engineering, 15, 871–879.

    Google Scholar 

  10. Georgiou, G., Stephens, D. L., Stathopoulos, C., Poetschke, H. L., Mendenhall, J., & Earhart, C. F. (1996). Protein Engineering, 9, 239–247.

    Article  CAS  Google Scholar 

  11. Ghasemi, S. H., Ahmadian, G., Sadeghi, M., Zeigler, D. R., Rahimian, H., Ghandili, S., et al. (2011). Enzyme and Microbial Technology, 48, 225–231.

    Article  CAS  Google Scholar 

  12. Itoh, Y., Watanabe, J., Fukada, H., Mizuno, R., Kezuka, Y., Nonaka, T., et al. (2006). Applied Microbiology and Biotechnology, 72, 1176–1184.

    Article  CAS  Google Scholar 

  13. Kuroda, K., & Ueda, M. (2003). Applied Microbiology and Biotechnology, 63, 182–186.

    Article  CAS  Google Scholar 

  14. Kuroda, K., & Ueda, M. (2006). Applied Microbiology and Biotechnology, 70, 458–463.

    Article  CAS  Google Scholar 

  15. Mauro, J. M., & Pazirandeh, M. (2000). Letters in Applied Microbiology, 30, 161–166.

    Article  CAS  Google Scholar 

  16. Pazirandeh, M., Wells, B. M., & Ryan, R. L. (1998). Applied and Environmental Microbiology, 64, 4068–4072.

    CAS  Google Scholar 

  17. Robinson, N. J., Gupta, A., Fordam-Skelton, A. P., Croy, R. R. D., Whitton, B. A., & Huckle, J. W. (1990). Proceedings of the Royal Society London B, 242, 241–247.

    Article  CAS  Google Scholar 

  18. Shi, J., Lindsay, W. P., Huckle, J. W., Morby, A. P., & Robinson, N. J. (1992). FEBS Letter, 303, 159–163.

    Article  CAS  Google Scholar 

  19. Shimizu, T., Hiyama, T., Ikeuchi, M., & Inoue, Y. (1992). Plant Molecular Biology, 20, 565–567.

    Article  CAS  Google Scholar 

  20. Sousa, C., Kotrba, P., Ruml, T., Cebolla, & Lorenzo, V. D. (1998). Journal of Bacteriology, 180, 2280–2284.

    CAS  Google Scholar 

  21. Suciu, I., Cosma, C., Todică, M., Bolboacă, S. D., & Jäntschi, L. (2008). International Journal of Molecular Sciences, 9, 434–453.

    Article  CAS  Google Scholar 

  22. Valls, M., González-Duarte, R., Atrian, S., & Lorenzo, V. D. (1998). Biochimie, 80, 855–861.

    Article  CAS  Google Scholar 

  23. Vijayaraghavan, K., & Yun, Y. (2008). Biotechnology Advances, 26, 266–291.

    Article  CAS  Google Scholar 

  24. Wang, J., & Chen, C. (2009). Biotechnology Advances, 27, 195–226.

    Article  Google Scholar 

  25. Wang, J. U., & Chao, Y. P. (2006). Applied and Environmental Microbiolog, 72, 927–931.

    Article  CAS  Google Scholar 

  26. Wu, C. H., Mulchandani, A., & Chen, W. (2008). Trends in Microbiology, 16, 181–188.

    Article  CAS  Google Scholar 

  27. Xu, Z., Bae, W., Mulchandani, A., Mehra, R. K., & Chen, W. (2002). Biomacromolecules, 3, 462–465.

    Article  CAS  Google Scholar 

  28. Yang, C., Zhu, Y., Yang, J., Liu, Z., Qiao, C., Mulchandani, A., et al. (2008). Applied and Environmental Microbiolog, 74, 7733–7739.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the National Institute of Genetic Engineering and Biotechnology (NIGEB) of Iran for support of this project. Funding for the project was provided by a grant from the National Institute for Genetic Engineering and Biotechnology to project number 341.

Author information

Authors and Affiliations

  1. Extremophiles Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran

    Vida Tafakori & Mohammad Ali Amoozegar

  2. Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd, Tehran, P.O. Box 14965/161, Iran

    Vida Tafakori & Gholamreza Ahmadian

Authors
  1. Vida Tafakori
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gholamreza Ahmadian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammad Ali Amoozegar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Gholamreza Ahmadian or Mohammad Ali Amoozegar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tafakori, V., Ahmadian, G. & Amoozegar, M.A. Surface Display of Bacterial Metallothioneins and a Chitin Binding Domain on Escherichia coli Increase Cadmium Adsorption and Cell Immobilization. Appl Biochem Biotechnol 167, 462–473 (2012). https://doi.org/10.1007/s12010-012-9684-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9684-x

Keywords

Search

Navigation