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Effects of Light Intensity on the Growth and Lipid Accumulation of Microalga Scenedesmus sp. 11-1 Under Nitrogen Limitation

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Abstract

Scenedesmus spp. have been reported as potential microalgal species used for the lipid production. This study investigated the effects of light intensity (at three levels: 50, 250, and 400 μmol photons m−2 s−1) on the growth and lipid production of Scenedesmus sp. 11-1 under N-limited condition. Carotenoid to chlorophyll ratio was higher when algae 11-1 grew under 250 and 400 μmol photons m−2 s−1 than that under 50 μmol photons m−2 s−1, while protein contents was lower. Highest biomass yield (3.88 g L−1), lipid content (41.1 %), and neutral lipid content (32.9 %) were achieved when algae 11-1 grew at 400 μmol photons m−2 s−1. Lipid production was slight lower at 250 μmol photons m−2 s−1 level compared to 400 μmol photons m−2 s−1. The major fatty acids in the neutral lipid of 11-1 were oleic acid (43–52 %), palmitic acid (24–27 %), and linoleic acid (7–11 %). In addition, polyunsaturated fatty acids had a positive correlation with total lipid production, and monounsaturated fatty acids had a negative one.

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Acknowledgment

This study was supported by grant KGCX2-YW-374-3 from Chinese Academy of Sciences and grant 2008ZX07422-003-5-2 from the Ministry of Science and Technology of China.

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  1. Junhan Liu

    Present address: Sinopharm Chemical Reagent Co., Ltd, Shanghai, 20002, China

Authors and Affiliations

  1. Key laboratory of Biofuels, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China

    Junhan Liu, Cheng Yuan, Guangrong Hu & Fuli Li

  2. Ocean College of Hebei Agricultural University, Qinhuangdao, 066003, China

    Cheng Yuan

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  1. Junhan Liu
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  2. Cheng Yuan
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  3. Guangrong Hu
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  4. Fuli Li
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Correspondence to Fuli Li.

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Liu, J., Yuan, C., Hu, G. et al. Effects of Light Intensity on the Growth and Lipid Accumulation of Microalga Scenedesmus sp. 11-1 Under Nitrogen Limitation. Appl Biochem Biotechnol 166, 2127–2137 (2012). https://doi.org/10.1007/s12010-012-9639-2

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  • DOI: https://doi.org/10.1007/s12010-012-9639-2

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