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Xylanase Isozymes from the Newly Isolated Bacillus sp. CKBx1D and Optimization of Its Deinking Potentiality

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Abstract

Recycling of civic paper waste by enzyme-based technology is nowadays a point of much concern for pollution-less green environment. In this study, the deinking effectiveness of purified xylanase from a newly isolated bacterium was evaluated for recycling of laser jet paper waste. A potent xylanases-producing bacterium from the microbial consortia of termite gut was isolated, which was further identified on the basis of 16S rRNA sequence as Bacillus sp. CKBx1D. In submerged fermentation condition, the isolate produced the highest level of xylanase (480 U/ml) at 36 h of growth. The extracellular xylanase system comprises of three distinct isozymes (est. Mw 35.28, 28.63, 18.94 kDa). The deinking of laser printed paper waste was performed using the purified enzyme mixture. Whole operational parameters were optimized using the Response Surface Methodology; it was found that at pH 6.8 with 47.2 h of continuous shaking at constant temperature of 35 °C, enzymes showed best deinking activity. After enzyme treatment, the physical properties of the pulp like brightness and ERIC (effective residual ink content) values were enhanced, whereas the pulp opacity was more reduced than the control treatment. Hence, the bacterial isolate and its xylanolytic enzyme system could efficiently be used in recycling paper waste as deinking agent.

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Acknowledgements

The authors are grateful to the Council of Scientific and Industrial Research (CSIR), Govt. of India, New Delhi for financial assistance to carry out this work. We are also thankful to Nandadulal Bhattacharyya, Principal, Vidyasagar Institute of Health (VIH), Midnapore for the meticulous revision of the paper.

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  1. Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Chiranjit Maity, Kuntal Ghosh, Suman K. Halder, Arijit Jana, Atanu Adak, Pradeep K. Das Mohapatra, Bikas R. Pati & Keshab C. Mondal

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  1. Chiranjit Maity
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Correspondence to Keshab C. Mondal.

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Maity, C., Ghosh, K., Halder, S.K. et al. Xylanase Isozymes from the Newly Isolated Bacillus sp. CKBx1D and Optimization of Its Deinking Potentiality. Appl Biochem Biotechnol 167, 1208–1219 (2012). https://doi.org/10.1007/s12010-012-9556-4

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