Abstract
Coffee is one of the most important agricultural products in Brazil. More than 50 % of the coffee fruit is not used for the production of commercial green coffee and is therefore discarded, usually ending up in the environment. The goal of this work was to select an efficient process for obtaining coffee pulp extract and to evaluate the use of this extract in bioethanol production. The effects of heat treatment and trituration on the yield and composition of the extract were investigated by measuring the amounts of reducing sugars, starch, pectin, and phenolic compounds. The extraction process was most efficient at room temperature using grinding followed by pressing. Five different fermentation media were tested: sugarcane juice or molasses diluted with water or with coffee pulp extract and a medium with only coffee pulp extract. Batch fermentations were carried out at 30 °C for 24 h, and samples were taken to obtain measurements of the total reducing sugars, cell count, and ethanol concentration. The addition of coffee pulp extract did not influence the fermentation or yeast viability, and it can thus be mixed with sugarcane juice or molasses for the production of bioethanol, with a yield of approximately 70 g/L.
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Acknowledgments
The authors are grateful to CNPq for granting the E.G.T.M. scholarship, to the Embrapa-Coffee covenant, and to UFLA for financial support of the project. The authors thank the Department of Biology and, in particular, Dr. Rosane Freitas Schwan, for providing the yeast, and the Lusiania alcohol factory for kindly providing the molasses.
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Menezes, E.G.T., do Carmo, J.R., Menezes, A.G.T. et al. Use of Different Extracts of Coffee Pulp for the Production of Bioethanol. Appl Biochem Biotechnol 169, 673–687 (2013). https://doi.org/10.1007/s12010-012-0030-0
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DOI: https://doi.org/10.1007/s12010-012-0030-0