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Preliminary Studies for Cephamycin C Purification Technique

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An Erratum to this article was published on 07 December 2011

Abstract

A study was made for purification of cephamycin C from fermentation of Streptomyces clavuligerus. Initially, the culture broth was clarified by microfiltration and ultrafiltration, after which the resulting permeates were subjected to nonspecific adsorption and ion-exchange chromatography on resin columns. The antibiotic activity was measured by the biological method at each stage by assaying its activity against the Escherichia coli ESS, super sensitive to β-lactam antibiotic. The purification processes were assessed in relation to the variables affecting each step. The purification efficiency by nonspecific adsorption was monitored by UV spectrophotometry, while the ion-exchange adsorption fractions were assessed by NMR spectroscopy. Some of the fractions obtained during purification were also analyzed by mass spectrometry (LC/MS and LC/MS/MS) to identify the cephamycin C molecule. These preliminary results proved the process feasibility.

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Abbreviations

Rt:

Retention times

1HNMR:

Proton nuclear magnetic resonance spectroscopy

ESI+:

Positive-mode electrospray ionization

TIC:

Total ion-current chromatogram

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Acknowledgements

The authors gratefully acknowledge the financial support of FAPESP (Grants 04/16056-6 and 05/55079-4).

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Authors and Affiliations

  1. Department of Chemical Engineering, Universidade Federal de São Carlos, Cx. Postal 676, CEP 13565-905, São Carlos, SP, Brazil

    Maritza Catalina Condori Bustamante, Carolina Bellão, Alberto Colli Badino Junior & Carlos Osamu Hokka

  2. Department of Chemical Engineering, Universidade Federal do Triângulo Mineiro, Cx. Postal 676, CEP 13565-905, São Carlos, SP, Brazil

    Ana Cláudia Granato & Marlei Barboza

  3. Department of Pharmacy, Universidade Federal de Alfenas, Cx. Postal 676, CEP 13565-905, São Carlos, SP, Brazil

    Jaine Honorata Hortolan Luiz de Oliveira

  4. Verdartis Desenvolvimento Biotecnológico Ltda—ME, Rua dos Técnicos s/n sala 5, Incubadora Supera Campus USP, CEP 14040-900, Riberão Preto, SP, Brazil

    Álvaro de Baptista Neto

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  1. Álvaro de Baptista Neto
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  2. Maritza Catalina Condori Bustamante
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Corresponding author

Correspondence to Marlei Barboza.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s12010-011-9472-z

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de Baptista Neto, Á., Bustamante, M.C.C., de Oliveira, J.H.H.L. et al. Preliminary Studies for Cephamycin C Purification Technique. Appl Biochem Biotechnol 166, 208–221 (2012). https://doi.org/10.1007/s12010-011-9417-6

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  • DOI: https://doi.org/10.1007/s12010-011-9417-6

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