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Reduction of Enzyme Dosage by Oxygen Delignification and Mechanical Refining for Enzymatic Hydrolysis of Green Liquor-Pretreated Hardwood

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Abstract

In this study, a strategy to reduce enzyme dosage is evaluated by applying two post-treatments, oxygen delignification and mechanical refining. The sugar conversion for GL12 substrates was increased from 51.5% to 77.9% with post-treatments at the enzyme dosage of 10 FPU. When the amount of enzyme was reduced to 5 FPU with post-treatments, the conversion of 71.8% was obtained, which was significant higher than the conversion without any post-treatment using 10 FPU (51.5%). This clearly demonstrates the benefit of post-treatments that allows more than 50% of enzyme reduction at the same level of enzymatic conversion. Enzyme-accessible surface area and pore volume were evaluated by Simons' staining and DSC thermoporometry methods, and strong correlations were found with the sugar conversion.

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Acknowledgments

This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government [NRF-2009-352-F00021] and Wood-to-Ethanol Research Consortium at North Carolina State University.

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Authors and Affiliations

  1. Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, 27695, USA

    Bon-Wook Koo, Trevor H. Treasure, Hasan Jameel, Richard B. Phillips, Hou-min Chang & Sunkyu Park

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  1. Bon-Wook Koo
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  2. Trevor H. Treasure
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  3. Hasan Jameel
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  4. Richard B. Phillips
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  5. Hou-min Chang
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  6. Sunkyu Park
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Correspondence to Sunkyu Park.

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Koo, BW., Treasure, T.H., Jameel, H. et al. Reduction of Enzyme Dosage by Oxygen Delignification and Mechanical Refining for Enzymatic Hydrolysis of Green Liquor-Pretreated Hardwood. Appl Biochem Biotechnol 165, 832–844 (2011). https://doi.org/10.1007/s12010-011-9301-4

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  • DOI: https://doi.org/10.1007/s12010-011-9301-4

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