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Biosolubilization of Rock Phosphate by Three Stress-Tolerant Fungal Strains

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Abstract

Three stress-tolerant phosphate-solubilizing fungal strains identified as Aspergillus niger, Aspergillus japonicus, and Penicillium simplicissimum were isolated from wheat rhizospheric soil. The strains demonstrated different capabilities of phosphate solubilization in National Botanical Research Institute’s phosphate medium containing rock phosphate (RP) as sole phosphorus (P) source, and the solubilization of RP by P. simplicissimum was the most effective among these strains, followed by A. niger and A. japonicus. All the strains exhibited high levels of stress tolerance like 10∼45°C temperature, 4∼11 pH, 0∼3.5% NaCl, and 0∼35% PEG 10000. The strains also differed in their abilities to survive and release soluble P from RP under different stresses. A. niger showed significantly higher tolerance to temperature and pH over the other two strains. Higher amount of spores and content of soluble P in the medium were observed in the presence of 3.5% NaCl with P. simplicissimum, followed by A. niger and A. japonicus. P. simplicissimum could not solubilize RP in the presence of 35% PEG 10000, which exhibited the lowest tolerance to desiccation stress among the three strains.

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Acknowledgments

This research work was kindly supported by National Natural Science Foundation of China (No. 51004078), the Program for Excellent Talents of the Education Department of Hubei Province, China (No. Q20101504), and the Scientific Research foundation of WIT (No. 10105041).

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Authors and Affiliations

  1. Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Lab of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan, 430073, China

    Chunqiao Xiao, Ruan Chi, Xiaohong Li, Min Xia & Zhongwu Xia

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  1. Chunqiao Xiao
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  2. Ruan Chi
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  3. Xiaohong Li
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  4. Min Xia
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  5. Zhongwu Xia
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Correspondence to Ruan Chi.

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Xiao, C., Chi, R., Li, X. et al. Biosolubilization of Rock Phosphate by Three Stress-Tolerant Fungal Strains. Appl Biochem Biotechnol 165, 719–727 (2011). https://doi.org/10.1007/s12010-011-9290-3

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  • DOI: https://doi.org/10.1007/s12010-011-9290-3

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