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Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities

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Abstract

In order to select Actinobacteria that could improve plant growth and thus agricultural yield, we assessed different plant growth promoting (PGP) abilities of eight rock phosphate (RP)-solubilizing Actinomycete isolates originating from Moroccan phosphate mines. Six of these strains were able to grow on root exudates of the wheat plant as sole nutrient sources and were efficiently releasing soluble phosphate from RP. These strains also inhibited the growth of potentially phytopathogenic fungi, bacteria (Gram +/−) or yeasts. Five of these strains produced indoleacetic acid and four showed endophytic properties. When these strains were grown, in the presence of the wheat plant, in a synthetic minimum medium (SMM) containing insoluble RP as unique phosphate source or in soil experiment, the most active RP-solubilizing strains had the highest stimulatory effect on the production of plant biomass. The most efficient strain Streptomyces griseus-related strain (BH7), stimulated aerial growth of the plant more than 70% in test tubes and more than 30% in RP soil compared to the non-inoculated control treatment. This study demonstrated that our selected Actinomycete strains could be used for the development of novel, non-polluting; farming practices by entering in the formulation of novel biofertilizer and biocontrol products constituted by spores and/or mycelium of the ad hoc Actinobacteria in association with pulverized RP.

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Acknowledgments

This work was supported by a Franco-Moroccan cooperation (CMIFM Volubilis PAI No MA/04/109F) financed by the Foreign Affairs Ministries of France and Morocco as well as by the French CNRS, the University Paris Sud 11 (Orsay, France) and the University Cadi Ayyad (Marrakech, Morocco). I thank M.E.S.M. for the attribution of the bourse 03/032.

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  1. Laboratoire de Biologie et de Biotechnologie des Microorganismes, Département de Biologie, Faculté de Sciences Semlalia, Université Cadi Ayyad (UCAM), Avenue Prince My. Abdellah, BP 23 90, Marrakech, 40 000, Morocco

    Hanane Hamdali & Yedir Ouhdouch

  2. Institut de Génétique et Microbiologie, Université Paris XI, 91405, Orsay, France

    Hanane Hamdali & Marie Joëlle Virolle

  3. Laboratoire d’ Ecologie Végétale, sol et Environnement, Faculté de Sciences Semlalia, Université Cadi Ayyad (UCAM), Marrakech, Morocco

    Mohamed Hafidi

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  1. Hanane Hamdali
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Correspondence to Yedir Ouhdouch.

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Hamdali, H., Hafidi, M., Virolle, M.J. et al. Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities. World J Microbiol Biotechnol 24, 2565–2575 (2008). https://doi.org/10.1007/s11274-008-9817-0

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