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Cloning of Two Genes (LAT1,2) Encoding Specific l-Arabinose Transporters of the l-Arabinose Fermenting Yeast Ambrosiozyma monospora

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Abstract

We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.

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Acknowledgment

This work was supported by the Academy of Finland through the following programmes: an Academy Research Fellowship for P.R. and the Finnish Centre of Excellence in White Biotechnology—Green Chemistry.

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  1. VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1000, Fi-02044 VTT, Finland

    Ritva Verho, Merja Penttilä & Peter Richard

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  1. Ritva Verho
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  2. Merja Penttilä
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  3. Peter Richard
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Correspondence to Peter Richard.

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Verho, R., Penttilä, M. & Richard, P. Cloning of Two Genes (LAT1,2) Encoding Specific l-Arabinose Transporters of the l-Arabinose Fermenting Yeast Ambrosiozyma monospora . Appl Biochem Biotechnol 164, 604–611 (2011). https://doi.org/10.1007/s12010-011-9161-y

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  • DOI: https://doi.org/10.1007/s12010-011-9161-y

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