Summary
Cells ofCandida shehatae repressed by growth in glucose- or D-xylose-medium produced a facilitated diffusion system that transported glucose (K s±2 mM,V max±2.3 mmoles g−1 h−1),d-xylose (K s±125 mM,V max±22.5 mmoles g−1 h−1) and D-mannose, but neither D-galactose norl-arabinose.
Cells derepressed by starvation formed several sugar-proton symports. One proton symport accumulated 3-0-methylglucose about 400-fold and transported glucose (K s±0.12 mM,V max ± 3.2 mmoles g−1 h−1) andd-mannose, a second proton symport transportedd-xylose (K s± 1.0 mM,V max 1.4 mmoles g−1 h−1) andd-galactose, whilel-arabinose apparently used a third proton symport. The stoicheiometry was one proton for each molecule of glucose or D-xylose transported. Substrates of one sugar proton symport inhibited non-competitively the transport of substrates of the other symports.
Starvation, while inducing the sugar-proton symports, silenced the facilitated diffusion system with respect to glucose transport but not with respect to the transport of D-xylose, facilitated diffusion functioning simultaneously with thed-xylose-proton symport.
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Lucas, C., van Uden, N. Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae . Appl Microbiol Biotechnol 23, 491–495 (1986). https://doi.org/10.1007/BF02346066
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DOI: https://doi.org/10.1007/BF02346066