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Utilization of Horticultural Waste for Laccase Production by Trametes versicolor Under Solid-State Fermentation

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Abstract

Horticultural waste collected from a landscape company in Singapore was utilized as the substrate for the production of laccase under solid-state fermentation by Trametes versicolor. The effects of substrate particle size, types of inducers, incubation temperature and time, initial medium pH value, and moisture content on laccase production were investigated. The optimum productivity of laccase (8.6 U/g substrate) was achieved by employing horticultural waste of particle size greater than 500 μm and using veratryl alcohol as the inducer. The culture was at 30 °C for 7 days at moisture content of solid substrate of 85% and initial pH 7.0. The decolorization was also investigated in order to assess the degrading capability of the ligninolytic laccase obtained in the above-mentioned cultures. The decolorization degree of a model dye, phenol red, was around 41.79% in 72 h of incubation. By far, this is the first report on the optimization of laccase production by T. versicolor under solid-state fermentation using horticultural waste as the substrate.

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Acknowledgments

The authors are grateful for the financial support to this work from Singapore Totalisation Board and Ngee Ann Kongsi.

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  1. School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, 535 Clementi Road, Singapore, 599489, Singapore

    Fengxue Xin & Anli Geng

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  1. Fengxue Xin
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  2. Anli Geng
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Correspondence to Anli Geng.

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Xin, F., Geng, A. Utilization of Horticultural Waste for Laccase Production by Trametes versicolor Under Solid-State Fermentation. Appl Biochem Biotechnol 163, 235–246 (2011). https://doi.org/10.1007/s12010-010-9033-x

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  • DOI: https://doi.org/10.1007/s12010-010-9033-x

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