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High-Level Expression and Efficient Purification of Bioactive Swollenin in Aspergillus oryzae

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Abstract

The bioactivity of swollenin is beneficial to cellulose decomposition by cellulase despite the lack of hydrolytic activity itself. In order to improve the productivity of swollenin, the effects of culture conditions on the expression level in recombinant Aspergillus oryzae were investigated systematically. With regard to the bioactivity of swollenin, glycerin and peanut meal were the optimal carbon or nitrogen source, respectively. The highest level production of swollenin (50 mg L−1) was attained after 88 h cultivation with the initial pH of 5.6 in the culture medium. Then the soluble swollenin was effectively purified from the cultural supernatant by ammonium sulfate precipitation and cationic exchange chromatography with recovery yield of 53.2%. The purified swollenin was fully bioactive due to its strong synergistic activity with cellulose.

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (2009AA02Z206), the National Basic Research Program of China (2007CB707805), and Nature Science Foundation of China (20736008, 20676115).

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Authors and Affiliations

  1. Institute of Bioengineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China

    Meihua Wang, Jin Cai, Lei Huang & Zhinan Xu

  2. Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Meihua Wang, Zhengbin Lv & Yaozhou Zhang

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  1. Meihua Wang
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  2. Jin Cai
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  3. Lei Huang
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  4. Zhengbin Lv
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  5. Yaozhou Zhang
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  6. Zhinan Xu
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Correspondence to Lei Huang or Zhinan Xu.

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Wang, M., Cai, J., Huang, L. et al. High-Level Expression and Efficient Purification of Bioactive Swollenin in Aspergillus oryzae . Appl Biochem Biotechnol 162, 2027–2036 (2010). https://doi.org/10.1007/s12010-010-8978-0

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  • DOI: https://doi.org/10.1007/s12010-010-8978-0

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