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Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay

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Abstract

Cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy and large-scale cell culture. For better quality control, it is important to accurately measure the microencapsulated cell viability and proliferation in the culture. A number of assays have been used for this purpose, but limitations arise. In this study, we investigated the feasibility and reliability of resazurin as a cell growth indicator in microencapsulated culture system. According to the experiment data, there was a reversible, time- and dose-dependent growth inhibition as observed for resazurin application in encapsulated cells. A positive relationship was observed between reduction of resazurin and CHO cell number in microcapsule. Moreover, the resazurin assay provided an equivalent result to the commonly used MTT method in determining CHO cell proliferation in APA microcapsule with no notable influence on cell distribution and organization pattern. In conclusion, resazurin assay is offered as a simple, rapid and non-invasive method for in vitro microencapsulated cell viability and proliferation measurement.

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Acknowledgements

This work was supported by the National Natural Science Foundation under Grant number 30970885 and National Key Sci-Tech Special Project of China under Grant number 2008ZX10002-019. We thank the many colleagues and collaborators who have contributed to the development of this work.

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Authors and Affiliations

  1. Laboratory of Biomedical Material Engineering, Department of Science and Technology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People’s Republic of China

    Jing Xiao, Ying Zhang, Jianzheng Wang, Weiting Yu, Wei Wang & Xiaojun Ma

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Jing Xiao & Jianzheng Wang

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  1. Jing Xiao
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  2. Ying Zhang
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  3. Jianzheng Wang
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  5. Wei Wang
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Correspondence to Xiaojun Ma.

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Xiao, J., Zhang, Y., Wang, J. et al. Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay. Appl Biochem Biotechnol 162, 1996–2007 (2010). https://doi.org/10.1007/s12010-010-8975-3

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  • DOI: https://doi.org/10.1007/s12010-010-8975-3

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