Abstract
This work reports the application of a lipase in the 2-ethylhexyl palmitate esterification in a solvent-free system with an immobilized lipase (Lipozyme RM IM). A sequential strategy was used applying two experimental designs to optimize the 2-ethylhexyl palmitate production. An empirical model was then built so as to assess the effects of process variables on the reaction conversion. Afterwards, the operating conditions that optimized 2-ethylhexyl palmitate production were established as being acid/alcohol molar ratio 1:3, temperature of 70°C, stirring rate of 150 rpm, 10 wt.% of enzyme, leading to a reaction conversion as high as 95%. From this point, a kinetic study was carried out evaluating the effect of acid:alcohol molar ratio, the enzyme concentration and the temperature on product conversion. The results obtained in this step permit to verify that an excess of alcohol (acid to alcohol molar ratio of 1:6), relatively low enzyme concentration (10 wt.%) and temperature of 70°C, led to conversions next to 100%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Koblitz, M. G. B. (2003). Purificação e caracterização de lipase de Rhyzopus sp. e sua aplicação na síntese de monoacilgliceróis. PhD Thesis. Campinas: UNICAMP (in Portuguese).
Dalla-Vechia, R., Nascimento, M. G., & Soldi, B. V. (2004). Química Nova, 27, 623–630.
Pandey, A., Selvakumar, P., Soccol, C. R., & Nigam, P. (1999). Current Science, 77, 149–162.
Gandhi, N. N. J. (1997). Journal of the American Oil Chemists’ Society, 74, 621–634.
Hasan, F., Shan, A. A., & Hameed, A. (2006). Enzyme and Microbial Technology, 39, 235–251.
He, X. L., Chen, B. Q., & Tan, T. W. (2002). Journal of Molecular Catalysis B, 18, 333–339.
Tan, T., Chen, B. Q., & Ye, H. (2006). Biochemical Engineering Journal, 29, 41–45.
Kumar, R., Madras, G., & Modak, J. (2004). Industrial & Engineering Chemistry Research, 43, 1568–1573.
Güvenç, A., Kapucu, N., & Mehmetoglu, N. (2002). Process Biochemistry, 38, 379–386.
Santos, J. C., Bueno, T., Ros, P. C. M., & Castro, H. F. (2007). Journal of Chemical Technology and Biotechnology, 82, 956–961.
Chang, S. W., Shaw, J. F., Shieh, C. H., & Shieh, C. J. (2006). Journal of Agricultural and Food Chemistry, 54, 7125–7129.
Richetti, A., Leite, S. G. F., Antunes, O. A. C., Lerin, L. A., Dallago, R. M., Emmerich, D., et al. (2009). Bioprocess and Biosystems Engineering. doi:10.1007/s00449-009-0328-7.
Dörmo, N., Bélafi-bakó, K., Bartha, L., Ehrenstein, U., & Gubicza, L. (2004). Biochemical Engineering Journal, 21, 229–234.
Karra-Châabouni, M., Ghamghi, H., Bezzine, S., Rekik, A., & Gargouri, Y. (2006). Process Biochemistry, 41, 1692–1698.
Kristensen, J. B., Xu, X., & Mu, H. (2005). Journal of Agricultural and Food Chemistry, 53, 7059–7064.
Coteron, A., Martinez, M., & Aracil, J. (1998). Journal of the American Oil Chemists’ Society, 75, 657–661.
Nordisk, N. (1992). Characteristics of immobilized lipase in ester synthesis and effects of water and temperature in various reactions. Technical Report A-05948.
Acknowledgments
The authors thank AGROPALMA, CAPES, and CNPq for the financial support of this work and scholarships.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Richetti, A., Leite, S.G.F., Antunes, O.A.C. et al. Optimization of 2-ethylhexyl Palmitate Production Using Lipozyme RM IM as Catalyst in a Solvent-Free System. Appl Biochem Biotechnol 160, 2498–2508 (2010). https://doi.org/10.1007/s12010-009-8756-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-009-8756-z