Abstract
In order to improve yields and to reduce the cost of oxalate decarboxylase (OxDC, EC 4.1.1.2), the induction of OxDC in the white-rot fungus Trametes versicolor was studied in this work. OxDC was induced by addition of inorganic acids including hydrochloric acid, sulfuric acid, and phosphoric acid to culture media. The results showed that all the acids could enhance OxDC expression. The activity of the acid-induced OxDC rose continuously. All of the OxDC volumetric activities induced by the inorganic acids were higher than 20.0 U/L and were two times higher than that obtained with oxalic acid. OxDC productivity was around 4.0 U•L−1•day−1. The highest specific activity against total protein was 3.2 U/mg protein at day 8 after induction of sulfuric acid, and the specific activity against mycelial dry weight was 10.6 U/g at day 9 after induction of hydrochloric acid. The growth of mycelia was inhibited slightly when the pH values in culture media was around 2.5–3.0, while the growth was inhibited heavily when the pH was lower than 2.5.
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Acknowledgments
Project 30671650, with support from the National Natural Science Foundation of China, the SIDA-Swedish Research Links Programme (348-2006-6705), the Open Project Program of the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0526) are gratefully acknowledged.
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Zhu, C.X., Hong, F. Induction of an Oxalate decarboxylase in the Filamentous Fungus Trametes versicolor by Addition of Inorganic Acids. Appl Biochem Biotechnol 160, 655–664 (2010). https://doi.org/10.1007/s12010-009-8571-6
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DOI: https://doi.org/10.1007/s12010-009-8571-6