Abstract
Methanotrophs have promising applications in the epoxidation of some alkenes and some chlorinated hydrocarbons and in the production of a biopolymer, poly-β-hydroxybutyrate (poly-3-hydroxybutyrate; PHB). In contrast with methane monooxygenase (MMO) activity and ability of PHB synthesis of four kinds of methanotrophic bacteria Methylosinus trichosporium OB3b, M. trichosporium IMV3011, Methylococcus capsulatus HD6T, Methylomonas sp. GYJ3, and the mixture of the four kinds of strains, M. trichosporium OB3b is the highest of the four in the activity of propene epoxidation (10.72 nmol/min mg dry weight of cell [dwc]), the activity of naphthalene oxidation (22.7 mmol/mg dwc), and ability in synthesis of PHB(11% PHB content in per gram dry weight of cell in 84 h). It could be feasible to improve the MMO activity by mixing four kinds of methanotrophs. The MMO activity dramatically decreased when the cellular PHB accumulated in the second stage. The reason for this may be the dilution of the MMO system in the cells with increasing PHB contents. It has been found that the PHB contents at the level of 1–5% are beneficial to the cells for maintenance of MMO epoxidation activity when enough PHB have been accumulated. Moreover, it was also found that high particulate methane monooxygenase activity may contribute to the synthesis of PHB in the cell, which could be used to improve the yield of PHB in methanotrophs.
Similar content being viewed by others
References
Higgins, I. J., Best, D. J., Hammond, R. C., & Scott, D. (1981). Microbiological Reviews, 45, 556–590.
Xin, J.-Y., Cui, J.-R., Niu, J.-Z., Hua, S.-F., Xia, C.-G., Li, S.-B., & Zhu, L.-M. (2004a). Biocatalysis and Biotransformation, 22, 225–229. doi:10.1080/10242420412331283305.
Xin, J.-Y., Cui, J.-R., Niu, J.-Z., Hua, S.-F., Xia, C.-G., Li, S.-B., & Zhu, L.-M. (2004b). Biotechnology, 3, 67–71.
Shah, N. N., Hanna, M. L., & Taylor, R. T. (1996). Biotechnology and Bioengineering, 49, 161–171. doi:10.1002/(SICI)1097-0290(19960120)49:2<161::AID-BIT5>3.0.CO;2-O.
Hanson, R. S., & Hanson, T. E. (1996). Methanotrophic bacteria. Microbiological Reviews, 60, 439–471.
Park, S., Shah, N. N., Taylor, R. T., & Droege, M. W. (1992). Biotechnology and Bioengineering, 40, 151–157. doi:10.1002/bit.260400121.
Cornish, A., MacDonald, J., Burrows, K. J., King, T. S., Scott, D., & Higgins, I. J. (1985). Biotechnology Letters, 7, 319–324. doi:10.1007/BF01030278.
Davis, K., Cornish, A., & Higgins, I. J. (1987). Journal of General Microbiology, 133, 291–297.
Shah, N. N., Park, S., Taylor, R. T., & Droege, M. W. (1992). Biotechnology and Bioengineering, 40, 705–712. doi:10.1002/bit.260400609.
Stanley, S. H., Prior, S. D., Leak, D. J., & Dalton, H. (1983). Biotechnology Letters, 5, 487–492. doi:10.1007/BF00132233.
Shah, N. N., Hanna, M. L., Jackson, K. J., & Taylor, R. T. (1995). Biotechnology and Bioengineering, 45, 229–238. doi:10.1002/bit.260450307.
Matsunaga, T., Matsunaga, N., & Nishimura, S. (1985). Biotechnology and Bioengineering, 27, 1277–1281. doi:10.1002/bit.260270902.
Nakamura, N., Sulaswatty, A., Nishimura, S., & Matsunaga, T. (1992). Journal of Biotechnology, 26, 163–171. doi:10.1016/0168-1656(92)90004-S.
Okura, I., Otsuka, K., Nakada, N., & Hasumi, F. (1990). Applied Biochemistry and Biotechnology, 24/25, 425–430. doi:10.1007/BF02920266.
Wong, C.-H., Daniels, L., Orme-Johnson, W. H., & Whitesides, G. M. (1981). Journal of the American Chemical Society, 103, 6227–6228. doi:10.1021/ja00410a049.
Stanley, S. H., & Dalton, H. (1992). Biocatalysis, 6, 163–175. doi:10.3109/10242429209014893.
Asenjo, J. A., & Suk, J. S. (1986). Journal of Fermentation Technology, 64, 271–278. doi:10.1016/0385-6380(86)90118-4.
Chang, H.-L., & Alvarez-Cohen, L. (1995). Biotechnology and Bioengineering, 45, 440–449. doi:10.1002/bit.260450509.
Henry, S. M., & Grbic-Galic, D. (1991). Applied and Environmental Microbiology, 57, 236–244.
Henrysson, T., & McCarty, P. L. (1993). Applied and Environmental Microbiology, 59, 1602–1606.
Oldenhuis, R., Oedzes, J. Y., Waarde, J., & Janssen, D. B. (1991). Applied and Environmental Microbiology, 57, 7–14.
Shen, R.-N., Yuchi, L., Ma, Q.-Q., & Li, S.-B. (1997). Archives of Biochemistry and Biophysics, 345, 223–229. doi:10.1006/abbi.1997.0239.
Park, S., Hanna, M. L., Taylor, R. T., & Droege, M. W. (1991). Biotechnology and Bioengineering, 38, 423–433. doi:10.1002/bit.260380412.
Brusseau, G. A., Tsien, H. C., Hanson, R. S., & Wackett, L. P. (1990). Biodegradation, 1, 19–29. doi:10.1007/BF00117048.
Chu, K. H., & Alvarez-Cohen, L. (1998). Applied and Environmental Microbiology, 64, 3451–3457.
Riis, V., & Mai, W. (1988). Journal of Chromatography, 445, 285–289. doi:10.1016/S0021-9673(01)84535-0.
Korotkova, N., & Lidstrom, M. E. (2001). Journal of Bacteriology, 18, 1038–1046. doi:10.1128/JB.183.3.1038-1046.2001.
Xin, J.-Y., Zhang, Y.-X., Zhang, S., Xia, C.-G., & Li, S.-B. (2007). Journal of Basic Microbiology, 47, 426–435. doi:10.1002/jobm.200710313.
Chu, K. H., & Alvarez-Cohen, L. (1996). Water Environment Research, 68, 76–82. doi:10.2175/106143096X127235.
Acknowledgements
The authors acknowledge the financial support of the work by the Program for New Century Excellent Talents in University (NCET-05-0358), the National Natural Science Foundation of China (20625308), and the Chinese Academy of Sciences.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Zhang, Y., Xin, J., Chen, L. et al. The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs. Appl Biochem Biotechnol 157, 431–441 (2009). https://doi.org/10.1007/s12010-008-8447-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8447-1