Skip to main content
SpringerLink
Log in

Lime Pretreatment of Sugarcane Bagasse for Bioethanol Production

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The pretreatment of sugarcane bagasse with lime (calcium hydroxide) is evaluated. The effect of lime pretreatment on digestibility was studied through analyses using central composite design (response surface), considering pretreatment time, temperature, and lime loading as factors. The responses evaluated were the yield of glucose from pretreated bagasse after enzymatic hydrolysis. Experiments were performed using the bagasse as it comes from an alcohol/sugar factory (non-screened bagasse) and bagasse in the size range from 0.248 to 1.397 mm (screened bagasse) (12-60 mesh). It was observed that the particle size presented influence in the release of fermentable sugars after enzymatic hydrolysis using low loading of cellulase and β-glucosidase (3.5 FPU/g dry pretreated biomass and 1.0 IU/g dry pretreated biomass, respectively).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Mojovic, L., Nikolic, S., Rakin, M., & Vukasinovic, M. (2006). Fuel, 85, 1750–1755. doi:10.1016/j.fuel.2006.01.018.

    Article  CAS  Google Scholar 

  2. Saha, B. C & Hayashi, K. (2004). Washington, DC: American Chemical Society, 2–34.

  3. Laser, M., Schulman, D., Allen, S. G., Lichwa, J., Antal Jr, M. J., & Lynd, L. R. (2002). Bioresource Technology, 81, 33–44. doi:10.1016/S0960-8524(01)00103-1.

    Article  CAS  Google Scholar 

  4. Lee, J. (1997). Journal of Biotechnology, 56, 1–24. doi:10.1016/S0168-1656(97)00073-4.

    Article  CAS  Google Scholar 

  5. Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y. Y., Holtzapple, M., et al. (2005). Bioresource Technology, 96, 673–686. doi:10.1016/j.biortech.2004.06.025.

    Article  CAS  Google Scholar 

  6. Wyman, C. E., Dale, B. E., Elander, R. T., Holtzapple, M., Ladisch, M. R., & Lee, Y. Y. (2005a). Bioresource Technology, 96, 1959–1966. doi:10.1016/j.biortech.2005.01.010.

    Article  CAS  Google Scholar 

  7. Wyman, C. E., Dale, B. E., Elander, R. T., Holtzapple, M., Ladisch, M. R., & Lee, Y. Y. (2005b). Bioresource Technology, 96, 2026–2032. doi:10.1016/j.biortech.2005.01.018.

    Article  CAS  Google Scholar 

  8. Wooley, R., Ruth, M., Glassner, D., & Sheehan, J. (1999). Biotechnology Progress, 15, 794–803. doi:10.1021/bp990107u.

    Article  CAS  Google Scholar 

  9. Chang, V. S., & Holtzapple, M. T. (2000). Applied Biochemistry and Biotechnology, 84–86, 1–37. doi:10.1385/ABAB:84-86:1-9:5.

    Google Scholar 

  10. Lesoing, G., Klopfenstein, T., Rush, I., & Ward, J. (1981). Journal of Animal Science, 51, 263.

    Google Scholar 

  11. Verma, M. L. (1983). In G. R. Pearce (Ed.), Canberra, ACT, Australia: Australian Government Publishing Service, 85–99.

  12. Playne, M. J. (1984). Biotechnology and Bioengineering, 26, 426–433. doi:10.1002/bit.260260505.

    Article  CAS  Google Scholar 

  13. Nagwani, M. (1992). M.S. thesis, Texas A&M University.

  14. NREL (National Renewable Energy Laboratory—EUA). (1996)

  15. Chang, V. S., Burr, B., & Holtzapple, M. T. (1997). Applied Biochemistry and Biotechnology, 63–65, 3–19. doi:10.1007/BF02920408.

    Article  Google Scholar 

  16. Chang, V. S., Nagwani, M., & Holtzapple, M. T. (1998). Applied Biochemistry and Biotechnology, 74, 135–159. doi:10.1007/BF02825962.

    Article  CAS  Google Scholar 

  17. Holtzapple, M. T., & Davison, R. R. (1999). Int. CI. C 13K1/02. US, PI 5,865,898.

  18. Kaar, W. E., & Holtzapple, M. T. (2000). Biomass Bioengineering, 18, 189–199. doi:10.1016/S0961-9534(99)00091-4.

    Article  CAS  Google Scholar 

  19. Kim, S., & Holtzapple, M. T. (2005). Bioresource Technology, 96(18), 1994–2006. doi:10.1016/j.biortech.2005.01.014.

    Article  CAS  Google Scholar 

  20. Salazar, R. F. S., Silva, G. L. P., & Silva, M. L. C. P. (2005). VI Congresso Brasileiro de Engenharia Química em Iniciação Científica, UNICAMP.

  21. Ferraz, A., Baeza, J., Rodriguez, J., & Freer, J. (2000). Bioresource Technology, 74(3), 201–212. doi:10.1016/S0960-8524(00)00024-9.

    Article  CAS  Google Scholar 

  22. Sluiter, A. (2006). Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory (NREL) Analytical Procedures, 1–14.

  23. Lin, Y. L., & Dence, C. W. (1992). Methods in lignin chemistry pp. 33–62. Berlin: Springer.

    Google Scholar 

  24. Irick, T. J., West, K., Brownell, H. H., Schiwald, W., & Saddler, J. N. (1988). Applied Biochemistry and Biotechnology, 17, 137–149. doi:10.1007/BF02779152.

    Article  CAS  Google Scholar 

  25. Kaar, W. E., & Brink, D. L. (1991). Journal of Wood Chemistry and Technology, 11, 479–494. doi:10.1080/02773819108051088.

    Article  CAS  Google Scholar 

  26. Kaar, W. E., Gool, L. G., Merriman, M. M., & Brink, D. L. (1991). Journal of Wood Chemistry and Technology, 11, 447–463. doi:10.1080/02773819108051086.

    Article  CAS  Google Scholar 

  27. Laver, M. L., & Wilson, K. P. (1993). Tappi Journal, 76(6), 155–159.

    CAS  Google Scholar 

  28. Szczodrak, J., & Fiedurek, J. (1996). Biomass and Bioenergy, 10(5/6), 367–375. doi:10.1016/0961-9534(95)00114-X.

    Article  CAS  Google Scholar 

  29. Ghose, T. K. (1987). Pure and Applied Chemistry, 59(2), 257–268. doi:10.1351/pac198759020257.

    Article  CAS  Google Scholar 

  30. Adney, B. & Baker, J. (1996). Chemical analysis and testing task—laboratory analytical procedure. LAP-006.

  31. Wood, T. M., & Bhat, K. M. (1988). Methods in enzymology (vol. 160, pp. 87–116). San Diego: Academic.

    Google Scholar 

  32. Miller, G. L. (1959). Analytical Chemistry, 31(3), 426–428. doi:10.1021/ac60147a030.

    Article  CAS  Google Scholar 

  33. Barros Neto, B., Scarmin, I. S., & Bruns, R. E. (2003). 2 ed. Campinas, SP: Editora da UNICAMP.

Download references

Acknowledgments

The authors acknowledge Fapesp for financial support.

Author information

Authors and Affiliations

  1. School of Chemical Engineering, University of Campinas, UNICAMP, Campinas, Brazil

    Sarita C. Rabelo, Rubens Maciel Filho & Aline Carvalho Costa

Authors
  1. Sarita C. Rabelo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rubens Maciel Filho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aline Carvalho Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aline Carvalho Costa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rabelo, S.C., Filho, R.M. & Costa, A.C. Lime Pretreatment of Sugarcane Bagasse for Bioethanol Production. Appl Biochem Biotechnol 153, 139–150 (2009). https://doi.org/10.1007/s12010-008-8433-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-008-8433-7

Keywords

Search

Navigation