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Cell-Wall Composition and Accessibility to Hydrolytic Enzymes is Differentially Altered in Divergently Bred Switchgrass (Panicum virgatum L.) Genotypes

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Abstract

The aims of this study were to understand the genotypic variability in cell-wall composition and cell-wall accessibility to enzymes in select switchgrass plants obtained from two different populations derived from a base population of octaploid cultivars. Population C+3 was developed by three breeding generations for high digestibility and population C−1 developed by one generation of breeding for low digestibility. Above-ground biomass from 12 selected genotypes, three each with high or low digestibility within each population, was analyzed for their cell-wall aromatics and polysaccharides. The ratio of p-coumaric acid/ferulic acid was greater (P ≤ 0.05) for the high-lignin C−1 population over the low-lignin C+3 population, although the amounts of these two phenolics did not differ between populations. Combined values of guaiacyl + syringyl-lignin were consistently higher in genotypes from the C−1 population as compared to the genotypes from the C+3 population. Overall, p-coumaric acid was released by enzymes in greater amounts than ferulic acid in all these genotypes. Genotypes in the C−1 population exhibited lower dry weight loss as compared to the genotypes in the C+3 population after enzymatic digestion, suggesting changes in cell-wall architecture. Overall, our data highlight the phenotypic plasticity coded by the switchgrass genome and suggest that combining dry matter digestibility with other more specific cell-wall traits could result in genotypes with greater utility as bioenergy feedstocks.

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Acknowledgments

We thank Nathan Palmer, Ashley Hejny, Ashley Sternhagen, Megan Poppas, and Luanne L. Rigsby for technical assistance. The authors gratefully thank W. Herbert Morrison III, ARS-USDA (retired), for his contributions on chemical analyses. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Authors and Affiliations

  1. Grain, Forage, and Bioenergy Research Unit, USDA-ARS, Department of Agronomy and Horticulture, East Campus, USDA-ARS, 314 Biochemistry Hall, Lincoln, NE, 68583-0737, USA

    Gautam Sarath, Robert B. Mitchell & Kenneth P. Vogel

  2. Quality Assessment Research Unit, USDA-ARS, SAA, RBRRC, 950 College Station Road, Athens, GA, 30605, USA

    Danny E. Akin

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  1. Gautam Sarath
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  2. Danny E. Akin
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  3. Robert B. Mitchell
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  4. Kenneth P. Vogel
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Correspondence to Gautam Sarath.

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Sarath, G., Akin, D.E., Mitchell, R.B. et al. Cell-Wall Composition and Accessibility to Hydrolytic Enzymes is Differentially Altered in Divergently Bred Switchgrass (Panicum virgatum L.) Genotypes. Appl Biochem Biotechnol 150, 1–14 (2008). https://doi.org/10.1007/s12010-008-8168-5

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  • DOI: https://doi.org/10.1007/s12010-008-8168-5

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