Abstract
Thermophilic ethanol fermentation of wet-exploded wheat straw hydrolysate was investigated in a continuous immobilized reactor system. The experiments were carried out in a lab-scale fluidized bed reactor (FBR) at 70°C. Undetoxified wheat straw hydrolysate was used (3–12% dry matter), corresponding to sugar mixtures of glucose and xylose ranging from 12 to 41 g/l. The organism, thermophilic anaerobic bacterium Thermoanaerobacter BG1L1, exhibited significant resistance to high levels of acetic acid (up to 10 g/l) and other metabolic inhibitors present in the hydrolysate. Although the hydrolysate was not detoxified, ethanol yield in a range of 0.39–0.42 g/g was obtained. Overall, sugar efficiency to ethanol was 68–76%. The reactor was operated continuously for approximately 143 days, and no contamination was seen without the use of any agent for preventing bacterial infections. The tested microorganism has considerable potential to be a novel candidate for lignocellulose bioconversion into ethanol. The work reported here also demonstrates that the use of FBR configuration might be a viable approach for thermophilic anaerobic ethanol fermentation.
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Kim, S., & Dale, B. E. (2004). Biomass and Bioenergy, 26, 361–375.
Olsson, L., & Hahn-Hägerdal, B. (1996). Enzyme and Microbial Technology, 18, 312–331.
Palmqvist, E., & Hahn-Hägerdal, B. (2000). Bioresource Technology, 74, 25–33.
Von Sivers, M., Zacchi, G., Olsson, L., & Hahn-Hägerdal, B. (1994). Biotechnology Progress, 10, 555–560.
Dien, B. S., Cotta, M. A., & Jeffries, T. W. (2003). Applied Microbiology and Biotechnology, 63, 258–266.
Aristidou, A., & Penttila, M. (2000). Current Opinion in Biotechnology, 11, 187–198.
Saha, B. C. (2003). Journal of Industrial Microbiology and Biotechnology, 30, 279–291.
Zaldivar, J., Nielsen, J., & Olsson, L. (2001). Applied Microbiology and Biotechnology, 56, 17–34.
Moniruzzaman, M., Dien, B. S., Skory, C. D., Chen, Z. D., Hespell, R. B., Ho, N. W. Y., et al. (1997). World Journal of Microbiology and Biotechnology, 13, 341–346.
Klapatch, T. R., Hogsett, D. A. L., Baskaran, S., Pal, S., & Lynd, L. R. (1994). Applied Biochemistry and Biotechnology, 45–46, 209–223.
Lynd, L. R. (1989). In Advances in biochemical engineering/biotechnology (vol 38, pp. 1–52). New York: Springer.
Georgieva, T. I., Mikkelsen, M. J., & Ahring, B. K. (2007). Central European Journal of Biology, DOI 10.2478/s11535-007-0026-x.
Sommer, P., Georgieva, T., & Ahring, B. K. (2004). Biochemical Society Transactions, 32, 283–289.
Schmidt, J. E., & Ahring, B. K. (1999). Applied and Environmental Microbiology, 65, 1050–1054.
Desai, S. G., Guerinot, M. L., & Lynd, L. R. (2004). Applied Microbiology and Biotechnology, 65, 600–605.
Lynd, L. R., Baskaran, S., & Casten, S. (2001). Biotechnology Progress, 17, 118–125.
Hild, H. M., Stuckey, D. C., & Leak, D. J. (2003). Applied Microbiology and Biotechnology, 60, 679–686.
Hill, P. W., Klapatch, T. R., & Lynd, L. R. (1993). Biotechnology and Bioengineering, 42, 873–883.
Lynd, L. R., Weimer, P. J., van Zyl W. H, & Pretorius, I. S. (2002). Microbiology and Molecular Biology Reviews, 66, 506–577.
Georgieva, T., & Ahring, B. K. (2007). 29th Symposium on Biotechnology for Fuels and Chemicals, 51.
Zaldivar, J., Roca, C., Le Foll, C., Hahn-Hägerdal, B., & Olsson, L. (2005). Bioresource Technology, 96, 1670–1676.
Klinke, H. B., Thomsen, A. B., & Ahring, B. K. (2004). Applied Microbiology and Biotechnology, 66, 10–26.
Banat, I. M., Nigam, P., Singh, D., Marchant, R., & Mchale, A. P. (1998). World Journal of Microbiology and Biotechnology, 14, 809–821.
Hörmeyer, H. F., Tailliez, P., Millet, J., Girard, H., Bonn, G., Bobleter, O., et al. (1988). Applied Microbiology and Biotechnology, 29, 528–535.
Lynd, L. R., Grethlein, H. E., & Wolkin, R. H. (1989). Applied and Environmental Microbiology, 55, 3131–3139.
Kaur, H. R. P. (1989). Biological Wastes, 30, 301–308.
Saha, B. C., & Cotta, M. A. (2006). Biotechnology Progress, 22, 449–453.
Saha, B. C., Iten, L. B., Cotta, M. A., & Wu, Y. V. (2005). Process Biochemistry, 40, 3693–3700.
Nigam, J. N. (2001). Journal of Biotechnology, 87, 17–27.
Amartey, S. A., Leung, P. C. J., Baghaei-Yazdi, N., Leak, D. J., & Hartley, B. S. (1999). Process Biochemistry, 34, 289–294.
Alfani, F., Gallifuoco, A., Saporosi, A., Spera, A., & Cantarella, M. (2000) Journal of Industrial Microbiology and Biotechnology, 25, 184–192.
Felby, C., Klinke, H. B., Olsen, H. S., & Thomsen, A. B. (2003). Applications of Enzymes to Lignocellulosics, 855, 157–174.
Mohagheghi, A., Tucker, M., Grohmann, K., & Wyman, C. (1992). Applied Biochemistry and Biotechnology, 33, 67–81.
Ballesteros, M., Oliva, J. M., Negro, M. J., Manzanares, P., & Ballesteros, I. (2004). Process Biochemistry, 39, 1843–1848.
Szczodrak, J. (1988). Biotechnology and Bioengineering, 32, 771–776.
Baskaran, S., Ahn, H. J., & Lynd, L. R. (1995). Biotechnology Progress, 11, 276–281.
Burdette, D. S., Jung, S. H., Shen, G. J., Hollingsworth, R. I., & Zeikus, J. G. (2002). Applied and Environmental Microbiology, 68, 1914–1918.
Acknowledgements
We thank Novozymes A/S for providing enzymes Celluclast and Novozyme 188. We further thank Thomas Andersen and Gitte Hinz-Berg from BioCentrum for excellent technical help.
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Georgieva, T.I., Mikkelsen, M.J. & Ahring, B.K. Ethanol Production from Wet-Exploded Wheat Straw Hydrolysate by Thermophilic Anaerobic Bacterium Thermoanaerobacter BG1L1 in a Continuous Immobilized Reactor. Appl Biochem Biotechnol 145, 99–110 (2008). https://doi.org/10.1007/s12010-007-8014-1
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DOI: https://doi.org/10.1007/s12010-007-8014-1